|
| Status |
Public on May 18, 2021 |
| Title |
Ba/F3, SPAG9-JAK2(-), rep2 |
| Sample type |
RNA |
| |
|
| Source name |
non-induced Ba/F3 cells not expressing SPAG9-JAK2 (mock Ba/F3)
|
| Organism |
Mus musculus |
| Characteristics |
cell line: Ba/F3
cell type: interleukin-3-dependent murine pro-B cell line
|
| Treatment protocol |
Ba/F3 cells expressing SPAG9-JAK2 under doxycycline dependent manner were established, and SPAG9-JAK2 protein expression was induced using 500 ng/ml doxycycline.
|
| Growth protocol |
Ba/F3 cells transduced with SPAG9-JAK2 fusion protein by the Retro-X Tet-on Advanced Inducible System (Takara Bio) were washed twice with phosphate-buffered saline and seeded in triplicate with or without 500 ng/ml doxycycline.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted 48 hours later from Ba/F3 cells with or without expression of SPAG9-JAK2 using an RNeasy Mini Kit (Qiagen, Hilden, Germany), and the quality was checked on a NanoDrop 2000 (Thermo Fisher Scientific) and a 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA).
|
| Label |
biotin
|
| Label protocol |
cRNA target synthesis was carried out using a GeneChip 3’IVT PLUS Reagent Kit (Thermo Fisher Scientific).
|
| |
|
| Hybridization protocol |
Biotin-labeled cRNA was hybridized to a GeneChip Mouse Genome 430 2.0 Array (Thermo Fisher Scientific) following the standard protocols.
|
| Scan protocol |
Hybridized arrays were stained and scanned with a Genechip 3000 7G Scanner (Thermo Fisher Scientific) at the default settings.
|
| Description |
Gene expression data from non-induced Ba/F3 cells not expressing SPAG9-JAK2 (mock Ba/F3)
|
| Data processing |
Data were analyzed using Affymetrix Expression Console Software 1.4.1.
|
| |
|
| Submission date |
May 17, 2021 |
| Last update date |
May 18, 2021 |
| Contact name |
Azusa Mayumi |
| Organization name |
Kyoto Prefectural University of Medicine
|
| Department |
Pediatrics
|
| Street address |
465, Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
602-8566 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE174578 |
SPAG9-JAK2 gene expression array in Ba/F3 cells |
|
