|
| Status |
Public on Apr 16, 2010 |
| Title |
OG2 ESCs |
| Sample type |
RNA |
| |
|
| Source name |
cell culture of OG2 ESCs
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse embryonic stem cells
cell line: OG2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol, according to manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Ambion TotalPrep RNA Amplification Kit. RNA input: 400ng, IVT: 12h
|
| |
|
| Hybridization protocol |
According to manufacturer's instructions. Hybridization time: 18h
|
| Scan protocol |
Illumina iScan system using default settings
|
| Description |
N/A
|
| Data processing |
Cubic Spline normalization with background subtraction in BeadStudio.
|
| |
|
| Submission date |
Apr 14, 2010 |
| Last update date |
Apr 15, 2010 |
| Contact name |
Boris Greber |
| E-mail(s) |
boris.greber@mpi-muenster.mpg.de
|
| Organization name |
Max Planck Institute for Molecular Biomedicine
|
| Department |
Cell and Developmental Biology
|
| Street address |
Röntgenstraße 20
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE21343 |
Global gene expression profiles of mouse ESCs, disease-specific iPSCs, and gene-corrected iPSCs |
|
