|
Status |
Public on Apr 16, 2010 |
Title |
FAH gene-corrected iPSCs, line 7.1 |
Sample type |
RNA |
|
|
Source name |
cell culture of FAH gene-corrected iPSCs, line 7, subclone 1
|
Organism |
Mus musculus |
Characteristics |
cell type: gene-corrected induced pluripotent stem cells genotype: gene-corrected FAH(-/-)
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol, according to manufacturer's instructions
|
Label |
Cy3
|
Label protocol |
Ambion TotalPrep RNA Amplification Kit. RNA input: 400ng, IVT: 12h
|
|
|
Hybridization protocol |
According to manufacturer's instructions. Hybridization time: 18h
|
Scan protocol |
Illumina iScan system using default settings
|
Description |
N/A
|
Data processing |
Cubic Spline normalization with background subtraction in BeadStudio.
|
|
|
Submission date |
Apr 14, 2010 |
Last update date |
Apr 15, 2010 |
Contact name |
Boris Greber |
E-mail(s) |
boris.greber@mpi-muenster.mpg.de
|
Organization name |
Max Planck Institute for Molecular Biomedicine
|
Department |
Cell and Developmental Biology
|
Street address |
Röntgenstraße 20
|
City |
Münster |
ZIP/Postal code |
48149 |
Country |
Germany |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE21343 |
Global gene expression profiles of mouse ESCs, disease-specific iPSCs, and gene-corrected iPSCs |
|