|
| Status |
Public on Jul 01, 2010 |
| Title |
Palate_Adamts9+/-:bt/bt_Rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Secondary mouse palatal shelf tissue at embryonic stage E13.75
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Murine palatal shelf tissue
strain: C57BL/6
genotype/variation: Adamts9+/-:bt/bt
age: E13.75
|
| Biomaterial provider |
The mice were bred and crossed at the Cleveland Clinic's Biological Resource Unit. Ms. Courtney Nelson made the genetic crosses and euthanised the mice for this experiment.
|
| Treatment protocol |
No treatment of the tissue. They were directly taken for RNA after dissection.
|
| Growth protocol |
No growth protocol
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from the tissue samples using the RNeasy kit from Qiagen.
|
| Label |
Biotin
|
| Label protocol |
250 ng of RNA was reverse transcribed into cRNA and biotin-UTP labeled using the Illumina TotalPrep RNA Amplification Kit (Ambion, Austin, TX).
|
| |
|
| Hybridization protocol |
cRNA was quantified using a nanodrop spectrophotometer and the cRNA quality (size distribution) was further analyzed on a 1% agarose gel. cRNA was hybridized to the Illumina MouseRef-8 v2 Expression BeadChip using standard protocols (provided by Illumina, San Diego, CA). Individual hybridizations were performed on each sample, and no pooling of samples took place.
|
| Scan protocol |
Scan and Imaging procedures adhered strictly to standard protocols provided by Illumina, San Diego, CA.
|
| Description |
None
|
| Data processing |
The summarized data from the raw microarray data were log2 transformed and processed with force-positive background correction and quantile normalization. Quality control analyses were applied to detect the outlier samples.
|
| |
|
| Submission date |
Apr 15, 2010 |
| Last update date |
Apr 16, 2010 |
| Contact name |
Courtney Nelson |
| E-mail(s) |
nelsonc5@ccf.org
|
| Organization name |
Cleveland Clinic
|
| Department |
Department of Biomedical Engineering
|
| Lab |
Dr. Suneel Apte
|
| Street address |
9500 Euclid Avenue
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44195 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE21358 |
Comparison of 2 ADAMTS metalloproteases in closure of mouse palate - versican proteolysis in regulating palatal mesenchyme proliferation |
|
