|
| Status |
Public on Jun 08, 2021 |
| Title |
intestine 1month 30%CR |
| Sample type |
RNA |
| |
|
| Source name |
rat intestine
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
tissue: Small intestine
gender: Male
dietary restriction: 30% reduction
time: 1 month
|
| Growth protocol |
5-week-old rats were purchased from Japan SLC (Tokyo, Japan).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using RNeasy Plant Mini Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Gene Array Scanner.
|
| Description |
i-1m-30
Rats fed reduced amount of diet.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jun 07, 2021 |
| Last update date |
Jun 08, 2021 |
| Contact name |
Kenji Saito |
| E-mail(s) |
kkkj774@yahoo.co.jp
|
| Phone |
+81-3-5841-5114
|
| Organization name |
The Univeristy of Tokyo
|
| Department |
Department of Applied Biological Chemistry
|
| Lab |
Nutritional Biochemistry
|
| Street address |
1-1-1, yayoi
|
| City |
bunkyo-ku |
| ZIP/Postal code |
113-8657 |
| Country |
Japan |
| |
|
| Platform ID |
GPL341 |
| Series (2) |
| GSE176298 |
Mild food restriction and gene expression profile (intestine) |
| GSE176300 |
Mild food restriction and gene expression profile |
|
