|
| Status |
Public on Jun 10, 2021 |
| Title |
HNE-1 cells, NCsh |
| Sample type |
SRA |
| |
|
| Source name |
KIF2C negative control cell
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HNE-1
genotype: KIF2C negative control cell
|
| Treatment protocol |
HNE-1 cells were infected with the NCsh and KIF2Csh lentivirus particles with 5μg/mL polybrene for forty-eight hours
|
| Growth protocol |
HNE-1 cells were cultured in RPMI 1640 medium containing 50 units/mL penicillin, 50 mg/mL streptomycin, and 10% (v/v) FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The KIF2C knockdown cells and its corresponding negative control cells were collected, total RNA was extracted, and cDNA libraries were then constructed and subjected to RNA-seq analysis
RNA libraries were prepared for sequencing using standard MGI-G400/MGI-2000 protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
MGISEQ-2000RS |
| |
|
| Data processing |
Bowtie2 was used to align raw data
The bam files was quantify by RSEM
Genome_build: mm9
Supplementary_files_format_and_content: genes_fpkm_expression.xlsx
|
| |
|
| Submission date |
Jun 09, 2021 |
| Last update date |
Jun 10, 2021 |
| Contact name |
Chuntao Tao |
| Organization name |
Chongqing Medical University
|
| Street address |
China
|
| City |
Chongqing |
| State/province |
State... |
| ZIP/Postal code |
408500 |
| Country |
China |
| |
|
| Platform ID |
GPL30209 |
| Series (1) |
| GSE176471 |
Cell cycle dysregulation with overexpression of KIF2C/MCAK is a critical event in nasopharyngeal carcinoma |
|
| Relations |
| BioSample |
SAMN19643024 |
| SRA |
SRX11099999 |
