|
| Status |
Public on Nov 01, 2018 |
| Title |
E14-mES.1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse embryonic stem cell
|
| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic stem cell
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from tissues with TRIzol (Life Technologies, NY) reagent according to the manufacturer’s protocol. RNA quality and integrity were confirmed by electrophoresis on agarose gel stained with ethidium bromide
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA samples for hybridization were prepared according to Illumina’s recommended sample labeling procedure: 500 ng of total RNA was used for cDNA synthesis, followed by an amplification/labeling step (in vitro transcription) to synthesize biotin-labeled cRNA using the Illumina® TotalPrep RNA Amplification kit (Ambion Inc., Austin, TX). cRNA concentrations using RiboGreen were measured (Quant-iTTM RiboGreen® RNA assay kit; Invitrogen-Molecular Probes, ON, Canada) by the Victor3 spectrophotometer (PerkinElmer, CT) and cRNA quality was checked on a 1% agarose gel.
|
| |
|
| Hybridization protocol |
Labeled, amplified material (1,500 ng per array) was hybridized to a ver. 2 of the Illumina MouseRef-8 BeadChip (24K) according to the Manufacturer's instructions (Illumina, Inc., San Diego, CA). Array signals were developed by Amersham fluorolink streptavidin-Cy3 (GE Healthcare Bio-Sciences, Little Chalfont, UK) following the BeadChip manual.
|
| Scan protocol |
Arrays were scanned with an Illumina Bead array Reader confocal scanner (BeadStation 500GXDW; Illumina, Inc., San Diego, CA) according to the Manufacturer's instructions.
|
| Description |
To export to gene expression data based on unique probe id, we use Illumina BeadStudio software.
|
| Data processing |
Array data processing were performed on Illumina BeadStudio software. We normalized gene expression data using log2 transformatoin.
|
| |
|
| Submission date |
Apr 23, 2010 |
| Last update date |
Nov 01, 2018 |
| Contact name |
Seon-Kyu Kim |
| E-mail(s) |
seonkyu@kribb.re.kr
|
| Phone |
+82-42-879-8107
|
| Organization name |
Korea Research Institutue of Bioscience & Biotechnology
|
| Department |
Personalized Genomic Medicine Research Center
|
| Street address |
125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Korea
|
| City |
Daejeon |
| ZIP/Postal code |
305-806 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE21493 |
Gene expression study in skin fibroblasts |
|
