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Sample GSM537500 Query DataSets for GSM537500
Status Public on Dec 08, 2010
Title EpiSC OG2, rep2
Sample type RNA
 
Source name EpiSC-OG2
Organism Mus musculus
Characteristics cell type: epiblast stem cell (EpiSC)
potency: pluripotent
gender: female
Extracted molecule total RNA
Extraction protocol RNA samples to be analyzed on microarrays were prepared using Qiagen RNeasy columns with on-column DNA digestion.
Label biotin
Label protocol 500 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h).
 
Hybridization protocol Standard Illumina hybridization protocol. Purified and labelled cRNA was hybridized for 18 hrs onto MouseRef-8 v2 expression BeadChips (Illumina) according to the manufacturer's instructions. After washing, as recommended, chips were stained with streptavidin-Cy3 (GE Healthcare).
Scan protocol Standard Illumina scanning protocol. Chips were scanned using the iScan reader (Illumina) and accompanying software.
Description Biological replicate 2 of 2.
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was performed using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Apr 26, 2010
Last update date Dec 08, 2010
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE21516 Direct Reprogramming of Fibroblasts into Epiblast Stem Cells

Data table header descriptions
ID_REF
VALUE Quantile-normalized intensity

Data table
ID_REF VALUE
ILMN_1212607 3.136999
ILMN_1212612 2.622024
ILMN_1212619 3.64787
ILMN_1212628 2.759426
ILMN_1212632 4.586077
ILMN_1212636 11.179855
ILMN_1212637 8.97494
ILMN_1212645 4.542632
ILMN_1212648 6.956042
ILMN_1212653 2.683072
ILMN_1212672 8.341533
ILMN_1212682 3.422358
ILMN_1212683 1.900362
ILMN_1212685 2.034297
ILMN_1212692 5.647718
ILMN_1212693 3.007199
ILMN_1212695 2.235876
ILMN_1212698 1.913002
ILMN_1212702 2.678217
ILMN_1212703 4.969351

Total number of rows: 25697

Table truncated, full table size 550 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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