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Status
Public on Apr 29, 2010
Title
DC.4+.Sp.ST#8
Sample type
RNA
Source name
spleen
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
Liberase Blendzymes III, DNAse I digestion CD3e, CD19, GR1, NK1.1
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
DC.4+.Sp#8, spleen CD4+ dendritic cell
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Apr 27, 2010
Last update date
Jul 13, 2018
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Relations Reanalyzed by
GSE68399 Reanalyzed by
GSE74316 Reanalyzed by
GSE115450 Reanalyzed by
GSE117080
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
15.268
10344622
175.4206
10344624
361.9353
10344633
965.9935
10344637
231.9313
10344653
15.9891
10344658
209.8452
10344674
9.0401
10344679
44.8338
10344707
204.9521
10344713
208.3933
10344723
149.6716
10344725
39.2073
10344741
3083.3041
10344743
27.9113
10344750
1022.2686
10344772
33.517
10344789
209.5808
10344797
272.6141
10344799
224.2127
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM538250_EA07068_90311_MoGene_DC2.SP#8.CEL.gz
4.5 Mb
(ftp) (http) CEL
Processed data included within Sample table
