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Status
Public on Apr 29, 2010
Title
DC.8+.SLN#3
Sample type
RNA
Source name
Subcutaneous LNs
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
Blendzyme liberase III + DNase digestion CD3, CD19, NK1.1, GR1, Ter119 MACS depletion
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
DC.8+.SLN#3, skin draining LN CD8+ dendritic cell
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Apr 27, 2010
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Relations Reanalyzed by
GSE74316
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
18.0639
10344622
162.2103
10344624
295.9507
10344633
933.2682
10344637
181.9739
10344653
15.6702
10344658
121.7186
10344674
12.166
10344679
54.1956
10344707
153.9013
10344713
244.1078
10344723
110.1706
10344725
35.9092
10344741
2714.5117
10344743
26.9846
10344750
710.389
10344772
32.6452
10344789
154.0218
10344797
426.6369
10344799
203.1666
24922 423 Kbytes .Supplementary file Size Download File type/resource GSM538257_EA07068_91076_MoGene_DC1.LN#3.CEL.gz
4.0 Mb
(ftp) (http) CEL
Processed data included within Sample table
