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Sample GSM538258 Query DataSets for GSM538258
Status Public on Apr 29, 2010
Title DC.8+.Sp.ST#5
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: PI SA-PECy7
genetic background: C57BL/6J
phenotype markers: CD11b CD4 CD45 CD11c CD8a
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol collagenase, dispase, DNAse digestion CD3e, CD19, Gr1, NK1.1 MACS depletion
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description DC.8+.Sp#5, spleen CD8+ dendritic cell
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 27, 2010
Last update date Jul 13, 2018
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1
Relations
Reanalyzed by GSE68399
Reanalyzed by GSE74316
Reanalyzed by GSE115450
Reanalyzed by GSE117080

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 16.0994
10344622 210.8544
10344624 315.7321
10344633 748.3569
10344637 221.1032
10344653 16.9848
10344658 173.1879
10344674 9.3897
10344679 46.2012
10344707 170.7742
10344713 222.8096
10344723 130.3597
10344725 45.3098
10344741 3015.2115
10344743 31.9653
10344750 876.7577
10344772 41.5354
10344789 163.5443
10344797 366.3124
10344799 236.3847

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM538258_EA07068_87571_MoGene_DC1.SP#5.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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