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Status
Public on Apr 29, 2010
Title
DC.8+.Sp.ST#6
Sample type
RNA
Source name
spleen
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
collagenase, dispase, DNAse digestion CD3e, CD19, GR1, NK1.1 MACS depletion
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
DC.8+.Sp#6, spleen CD8+ dendritic cell
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Apr 27, 2010
Last update date
Jul 13, 2018
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Relations Reanalyzed by
GSE68399 Reanalyzed by
GSE74316 Reanalyzed by
GSE115450 Reanalyzed by
GSE117080
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
15.754
10344622
209.2325
10344624
336.0483
10344633
817.2896
10344637
258.0802
10344653
16.7279
10344658
168.1346
10344674
10.7016
10344679
40.6211
10344707
216.7664
10344713
266.4317
10344723
152.7004
10344725
42.8909
10344741
2763.4628
10344743
29.3427
10344750
914.3297
10344772
43.9768
10344789
209.123
10344797
341.7281
10344799
247.974
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM538259_EA07068_87572_MoGene_DC1.SP#6.CEL.gz
4.2 Mb
(ftp) (http) CEL
Processed data included within Sample table
