|
| Status |
Public on May 05, 2010 |
| Title |
Lymph node DCs + Papain for 4h |
| Sample type |
RNA |
| |
|
| Source name |
Lymph node
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: Lymph node CD11c+ DCs
agent: Papain
time: 4h
|
| Treatment protocol |
Lymph node CD11c+ DCs treated with Papain for 4h
|
| Growth protocol |
CD11c+ LN DCs were purified using the MACS-column from Miltenyi. Cells were cultured in the presence of 3T3-CD40L cells and treated with papain (25 µg/ml) for 4h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen RNeasy Mini Kit protocol according to the manufacturer’s instructions. Samples were treated with DNase
|
| Label |
Biotin
|
| Label protocol |
As per Vanderbilt Microarray Shared Resource Standard Protocol
|
| |
|
| Hybridization protocol |
As per Vanderbilt Microarray Shared Resource Standard Protocol
|
| Scan protocol |
As per Vanderbilt Microarray Shared Resource Standard Protocol
|
| Description |
Gene expression from mouse lymph node CD11c+ DCs treated with Papain for 4h
|
| Data processing |
For this analysis, we used Affymetrix Mouse Genome 430 2.0 Array (GPL1261), but instead of using Affymetrix’s sequence clusters to define genes, gene sequence clusters were RMA pre-processed and normalized with the UniGene Custom CDF Version 10 available fromhttp://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/CDF_download.asp to yield a list of 19,508 genes
|
| |
|
| Submission date |
Apr 29, 2010 |
| Last update date |
May 04, 2010 |
| Contact name |
Helder I. Nakaya |
| E-mail(s) |
hnakaya@usp.br
|
| Phone |
+551126481130
|
| Organization name |
Universidade de São Paulo
|
| Lab |
csbiology.com
|
| Street address |
AVENIDA PROFESSOR LINEU PRESTES, 580, Block 17
|
| City |
SÃO PAULO |
| State/province |
SÃO PAULO |
| ZIP/Postal code |
05508000 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL10369 |
| Series (1) |
| GSE21602 |
Microarray analyses of lymph node dendritic cells (DCs) stimulated in vitro with papain or LPS |
|
