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Status |
Public on Jun 23, 2021 |
Title |
reinal_organoid_wildtype_dd23 |
Sample type |
RNA |
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Source name |
retinal organoids
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Organism |
Mus musculus |
Characteristics |
genotype: wildtype time: differentiation day dd23 tissue: retinal organoids
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Treatment protocol |
Retinal organoids were harvested (5 per sample), homogenized. TRIZOL reagent was added and stored at -80°C until use.
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Growth protocol |
Retinal organoid were derived from mouse ES/iPS cell using the modified serum-free floating of embryoid-body-like aggregates with quick reaggregation method (SFEBq) (Eiraku et al., 2011, Assawachananont et al., 2014).
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was prepared using TRIZOL reagent (thermo fisher), following manufacturers instructions. Extracted RNA was purified using QIAGEN RNeasy Micro Kit and quantified using a NanoDrop-1000 spectrophotometer and the quality of purified RNA was monitored using Agilent 2100 Bioanalyzer.
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Label |
Cy3
|
Label protocol |
Labeling was conducted by Hokkaido System Corporation (Hokkaido, Japan), according to Agilent protocols, using the Low Input Quick Amp Labeling Kit (Agilent Technologies)
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|
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Hybridization protocol |
Hybridization was conducted by Hokkaido System Corporation (Hokkaido, Japan), according to Agilent protocols using the Gene Expression Hybridization Kit (Agilent Technologies)
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Scan protocol |
Scanning was conducted by Hokkaido System Corporation (Hokkaido, Japan), according to Agilent protocols, using Agilent Technologies Microarray Scanner
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Description |
Gene expression of DD23 wildtype retinal organoids NCTdd23
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Data processing |
Data was processed by Hokkaido System Corporation (Hokkaido, Japan) suing Agilent Feature Extraction 12.0.3.1. Data was normalized (75 percentile shift) using GeneSpring GX
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Submission date |
Jun 22, 2021 |
Last update date |
Jun 23, 2021 |
Contact name |
Takesi Matsuyama Hoyos |
E-mail(s) |
matsutakehoyo@gmail.com
|
Organization name |
RIKEN
|
Department |
BDR
|
Lab |
Retinal Regeneration Lab
|
Street address |
2-2-3 Minatojima-minamimachi, Chuou-ku
|
City |
Kobe |
State/province |
Hyogo |
ZIP/Postal code |
6500047 |
Country |
Japan |
|
|
Platform ID |
GPL21810 |
Series (1) |
GSE178653 |
Microarray analysis of mouse retinal organoids |
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