|
| Status |
Public on Jun 23, 2021 |
| Title |
reinal_organoid_wildtype_dd23 |
| Sample type |
RNA |
| |
|
| Source name |
retinal organoids
|
| Organism |
Mus musculus |
| Characteristics |
genotype: wildtype
time: differentiation day dd23
tissue: retinal organoids
|
| Treatment protocol |
Retinal organoids were harvested (5 per sample), homogenized. TRIZOL reagent was added and stored at -80°C until use.
|
| Growth protocol |
Retinal organoid were derived from mouse ES/iPS cell using the modified serum-free floating of embryoid-body-like aggregates with quick reaggregation method (SFEBq) (Eiraku et al., 2011, Assawachananont et al., 2014).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using TRIZOL reagent (thermo fisher), following manufacturers instructions. Extracted RNA was purified using QIAGEN RNeasy Micro Kit and quantified using a NanoDrop-1000 spectrophotometer and the quality of purified RNA was monitored using Agilent 2100 Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
Labeling was conducted by Hokkaido System Corporation (Hokkaido, Japan), according to Agilent protocols, using the Low Input Quick Amp Labeling Kit (Agilent Technologies)
|
| |
|
| Hybridization protocol |
Hybridization was conducted by Hokkaido System Corporation (Hokkaido, Japan), according to Agilent protocols using the Gene Expression Hybridization Kit (Agilent Technologies)
|
| Scan protocol |
Scanning was conducted by Hokkaido System Corporation (Hokkaido, Japan), according to Agilent protocols, using Agilent Technologies Microarray Scanner
|
| Description |
Gene expression of DD23 wildtype retinal organoids
NCTdd23
|
| Data processing |
Data was processed by Hokkaido System Corporation (Hokkaido, Japan) suing Agilent Feature Extraction 12.0.3.1. Data was normalized (75 percentile shift) using GeneSpring GX
|
| |
|
| Submission date |
Jun 22, 2021 |
| Last update date |
Jun 23, 2021 |
| Contact name |
Takesi Matsuyama Hoyos |
| E-mail(s) |
matsutakehoyo@gmail.com
|
| Organization name |
RIKEN
|
| Department |
BDR
|
| Lab |
Retinal Regeneration Lab
|
| Street address |
2-2-3 Minatojima-minamimachi, Chuou-ku
|
| City |
Kobe |
| State/province |
Hyogo |
| ZIP/Postal code |
6500047 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21810 |
| Series (1) |
| GSE178653 |
Microarray analysis of mouse retinal organoids |
|
