|
| Status |
Public on Sep 01, 2010 |
| Title |
DEHP, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
2-day-old rat hearts, DEHP
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
tissue: heart
age: 2 days
treatment: DEHP
treatment dosage: 50 ug/ml
treatment time: 3 days
|
| Treatment protocol |
Cells were treated for 3 days with either 50ug/mL DEHP (in 0.1% DMSO) or with vehicle control (0.1% DMSO).
|
| Growth protocol |
Cardiomyocytes isolated from 2-day-old Sprague-Dawley rat pups using an enzymatic digestion protocol. Cells were plated on laminated coverslips. Cells were cultured for 3 days prior to treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from cell monolayers using TRIzol reagent (Invitrogen) and total RNA was purified using RNeasy columns (Qiagen) according to manufacturer's protocols.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was generated according to the standard Affymetrix protocol from 5 ug of total RNA (GeneChip Expression Analysis Technical Manual; Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45C on the Rat 1.0 ST arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from cardiomyocyte monolayers treated with DEHP, 3 days.
|
| Data processing |
Data was exported from Affymetrix software (CEL files). Analysis was conducted using GeneSpring GX10. The GeneSpring software performed a baseline transformation, which takes the median of all samples. Quantile normalization (RMA) and summarization were then performed.
|
| |
|
| Submission date |
May 03, 2010 |
| Last update date |
May 17, 2010 |
| Contact name |
Nikki Gillum Posnack |
| E-mail(s) |
nposnack@childrensnational.org
|
| Phone |
202-4762475
|
| Organization name |
Childrens National Hospital
|
| Lab |
Posnack
|
| Street address |
111 Michigan Ave, NW
|
| City |
Washington |
| State/province |
Please select a region, state or province. |
| ZIP/Postal code |
20010 |
| Country |
USA |
| |
|
| Platform ID |
GPL6247 |
| Series (2) |
| GSE21640 |
Gene expression analysis of cardiomyocytes treated with a clinically relevant concentration of DEHP (50 ug/mL) |
| GSE21642 |
Genome-wide gene expression analysis of Di-(2-ethyl hexyl) phthalate-treated cardiomyocytes |
|
