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Sample GSM540779 Query DataSets for GSM540779
Status Public on Nov 01, 2011
Title Fibroblasts NIH-3T3_unused cardiomyocyte media_rep1
Sample type RNA
 
Source name Fibroblasts_untreated
Organism Mus musculus
Characteristics cell type: fibroblasts
cell line: NIH-3T3
Growth protocol Cultured cardiomyocytes and fibroblasts in claycomb medium in 37 degrees, 5% CO2
Extracted molecule total RNA
Extraction protocol Fibroblasts were put in RNAlater, Qiagen miRNEasy Mini Kit was used to extract total RNA.
Label Cy3
Label protocol Illumina TotalPrep RNA Amplification Kit was used
 
Hybridization protocol Performed according to Illumina protocol
Scan protocol Illumina Beadstation 500GX according to Illumina protocol
Description Chip sample 4936640003_A
Data processing Cubic spline normalization, subtracted background, False Discovery Rate.
Illumina Beadstudio 3.3 was used with gene expression module 3.4.0
 
Submission date May 05, 2010
Last update date Nov 01, 2011
Contact name Urban Hellman
E-mail(s) urban.hellman@medbio.umu.se
Phone +46907851621
Organization name Umea University
Department Public health and clinical medicine
Street address Nus 6M
City Umea
ZIP/Postal code 90185
Country Sweden
 
Platform ID GPL6885
Series (1)
GSE21676 Cultured fibroblasts treated with media from cardiomyocytes.

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_2896528 247.7484 53 16.75574 0
ILMN_2721178 70.24149 37 11.17034 0
ILMN_3033922 994.5928 42 53.19152 0
ILMN_3092673 4925.597 48 239.6291 0
ILMN_2816356 -4.904097 36 5.53013 0.7556391
ILMN_2808939 936.3339 35 60.2386 0
ILMN_2634564 448.6996 31 39.80078 0
ILMN_2737647 -9.679647 24 5.922459 0.9461153
ILMN_2734484 157.2339 39 14.37942 0
ILMN_2952292 91.03742 40 8.072549 0
ILMN_2699078 6.658937 38 5.749703 0.2155388
ILMN_1213681 177.4771 46 14.54073 0
ILMN_2735413 -6.245348 30 4.984612 0.8308271
ILMN_2735415 12.81764 32 6.574955 0.1002506
ILMN_2891688 391.6225 40 26.66974 0
ILMN_2637698 763.5116 44 40.9194 0
ILMN_2674228 323.2609 45 20.12088 0
ILMN_2601546 0.9404912 36 5.887018 0.4335839
ILMN_1230831 -6.379853 40 5.946 0.8345864
ILMN_2848071 2.274166 35 6.440369 0.3784461

Total number of rows: 25697

Table truncated, full table size 1043 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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