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Sample GSM540781 Query DataSets for GSM540781
Status Public on Nov 01, 2011
Title Fibroblasts NIH-3T3_unused cardiomyocyte media_rep3
Sample type RNA
 
Source name Fibroblasts_untreated
Organism Mus musculus
Characteristics cell type: fibroblasts
cell line: NIH-3T3
Growth protocol Cultured cardiomyocytes and fibroblasts in claycomb medium in 37 degrees, 5% CO2
Extracted molecule total RNA
Extraction protocol Fibroblasts were put in RNAlater, Qiagen miRNEasy Mini Kit was used to extract total RNA.
Label Cy3
Label protocol Illumina TotalPrep RNA Amplification Kit was used
 
Hybridization protocol Performed according to Illumina protocol
Scan protocol Illumina Beadstation 500GX according to Illumina protocol
Description Chip sample 4936640003_C
Data processing Cubic spline normalization, subtracted background, False Discovery Rate.
Illumina Beadstudio 3.3 was used with gene expression module 3.4.0
 
Submission date May 05, 2010
Last update date Nov 01, 2011
Contact name Urban Hellman
E-mail(s) urban.hellman@medbio.umu.se
Phone +46907851621
Organization name Umea University
Department Public health and clinical medicine
Street address Nus 6M
City Umea
ZIP/Postal code 90185
Country Sweden
 
Platform ID GPL6885
Series (1)
GSE21676 Cultured fibroblasts treated with media from cardiomyocytes.

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_2896528 341.1486 44 33.94194 0
ILMN_2721178 36.81084 48 7.341599 0.0112782
ILMN_3033922 1063.207 34 148.5755 0
ILMN_3092673 5130.371 43 469.1347 0
ILMN_2816356 -4.736936 25 5.386063 0.7531328
ILMN_2808939 1141.703 31 173.0744 0
ILMN_2634564 389.6616 32 59.35333 0
ILMN_2737647 -5.560986 42 5.102961 0.7844611
ILMN_2734484 253.0475 33 36.91127 0
ILMN_2952292 71.76054 40 11.12256 0
ILMN_2699078 1.820349 33 5.036818 0.4022556
ILMN_1213681 131.3537 44 20.70586 0
ILMN_2735413 -4.259257 34 6.644715 0.7218045
ILMN_2735415 3.264469 51 6.328625 0.3308271
ILMN_2891688 426.817 38 59.93161 0
ILMN_2637698 819.6437 48 87.54127 0
ILMN_2674228 291.0077 41 34.07988 0
ILMN_2601546 -3.041647 44 4.31298 0.6641604
ILMN_1230831 -13.07227 33 5.699604 0.9924812
ILMN_2848071 -6.083468 29 4.78556 0.8132832

Total number of rows: 25697

Table truncated, full table size 1046 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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