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Sample GSM540782 Query DataSets for GSM540782
Status Public on Nov 01, 2011
Title Fibroblasts NIH-3T3_media from cardiomyocytes_rep1
Sample type RNA
 
Source name Fibroblasts_media from cardiomyocytes
Organism Mus musculus
Characteristics cell type: fibroblasts
cell line: NIH-3T3
Growth protocol Cultured cardiomyocytes and fibroblasts in claycomb medium in 37 degrees, 5% CO2
Extracted molecule total RNA
Extraction protocol Fibroblasts were put in RNAlater, Qiagen miRNEasy Mini Kit was used to extract total RNA.
Label Cy3
Label protocol Illumina TotalPrep RNA Amplification Kit was used
 
Hybridization protocol Performed according to Illumina protocol
Scan protocol Illumina Beadstation 500GX according to Illumina protocol
Description Chip sample 4936640003_H
Data processing Cubic spline normalization, subtracted background, False Discovery Rate.
Illumina Beadstudio 3.3 was used with gene expression module 3.4.0
 
Submission date May 05, 2010
Last update date Nov 01, 2011
Contact name Urban Hellman
E-mail(s) urban.hellman@medbio.umu.se
Phone +46907851621
Organization name Umea University
Department Public health and clinical medicine
Street address Nus 6M
City Umea
ZIP/Postal code 90185
Country Sweden
 
Platform ID GPL6885
Series (1)
GSE21676 Cultured fibroblasts treated with media from cardiomyocytes.

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_2896528 248.2443 48 16.66552 0
ILMN_2721178 75.58466 41 8.459237 0
ILMN_3033922 1186.77 47 64.4269 0
ILMN_3092673 4357.438 60 154.2155 0
ILMN_2816356 -5.566393 42 5.004558 0.7769424
ILMN_2808939 1385.216 39 77.76473 0
ILMN_2634564 241.6252 31 19.28162 0
ILMN_2737647 -6.299699 38 5.338156 0.8157895
ILMN_2734484 157.6271 42 15.9916 0
ILMN_2952292 50.25794 63 5.783185 0
ILMN_2699078 2.166759 40 5.24094 0.3508772
ILMN_1213681 156.1852 51 12.90857 0
ILMN_2735413 2.552109 42 4.634587 0.3383459
ILMN_2735415 15.03736 36 6.601908 0.07017544
ILMN_2891688 321.691 49 22.01519 0
ILMN_2637698 624.114 38 33.40991 0
ILMN_2674228 356.2462 49 16.85155 0
ILMN_2601546 1.095122 43 5.549829 0.4047619
ILMN_1230831 -9.830066 37 5.297309 0.962406
ILMN_2848071 -5.769567 40 5.08887 0.7944862

Total number of rows: 25697

Table truncated, full table size 1042 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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