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Sample GSM540783 Query DataSets for GSM540783
Status Public on Nov 01, 2011
Title Fibroblasts NIH-3T3_media from cardiomyocytes_rep2
Sample type RNA
 
Source name Fibroblasts_media from cardiomyocytes
Organism Mus musculus
Characteristics cell type: fibroblasts
cell line: NIH-3T3
Growth protocol Cultured cardiomyocytes and fibroblasts in claycomb medium in 37 degrees, 5% CO2
Extracted molecule total RNA
Extraction protocol Fibroblasts were put in RNAlater, Qiagen miRNEasy Mini Kit was used to extract total RNA.
Label Cy3
Label protocol Illumina TotalPrep RNA Amplification Kit was used
 
Hybridization protocol Performed according to Illumina protocol
Scan protocol Illumina Beadstation 500GX according to Illumina protocol
Description Chip sample 4613963056_C
Data processing Cubic spline normalization, subtracted background, False Discovery Rate.
Illumina Beadstudio 3.3 was used with gene expression module 3.4.0
 
Submission date May 05, 2010
Last update date Nov 01, 2011
Contact name Urban Hellman
E-mail(s) urban.hellman@medbio.umu.se
Phone +46907851621
Organization name Umea University
Department Public health and clinical medicine
Street address Nus 6M
City Umea
ZIP/Postal code 90185
Country Sweden
 
Platform ID GPL6885
Series (1)
GSE21676 Cultured fibroblasts treated with media from cardiomyocytes.

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_2896528 199.4091 45 11.36423 0
ILMN_2721178 36.06055 51 6.412766 0.002506266
ILMN_3033922 1363.884 35 52.76126 0
ILMN_3092673 5442.84 52 165.5423 0
ILMN_2816356 0.4753602 41 2.680716 0.4348371
ILMN_2808939 931.9111 70 28.96121 0
ILMN_2634564 463.6501 33 30.30473 0
ILMN_2737647 -4.354846 39 2.516991 0.7280702
ILMN_2734484 163.4279 32 14.41726 0
ILMN_2952292 57.46428 52 5.721331 0
ILMN_2699078 -6.426123 46 2.141235 0.8308271
ILMN_1213681 109.0427 34 9.995558 0
ILMN_2735413 8.557055 28 3.813233 0.1353384
ILMN_2735415 19.12415 27 5.361933 0.01879699
ILMN_2891688 308.4038 36 17.55286 0
ILMN_2637698 747.1654 45 38.23114 0
ILMN_2674228 400.9895 42 20.42581 0
ILMN_2601546 0.6051368 43 3.067915 0.4260652
ILMN_1230831 1.804322 29 3.817204 0.3609022
ILMN_2848071 8.811722 28 4.251956 0.1253133

Total number of rows: 25697

Table truncated, full table size 1041 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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