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Sample GSM540784 Query DataSets for GSM540784
Status Public on Nov 01, 2011
Title Fibroblasts NIH-3T3_media from cardiomyocytes_rep3
Sample type RNA
 
Source name Fibroblasts_media from cardiomyocytes
Organism Mus musculus
Characteristics cell type: fibroblasts
cell line: NIH-3T3
Growth protocol Cultured cardiomyocytes and fibroblasts in claycomb medium in 37 degrees, 5% CO2
Extracted molecule total RNA
Extraction protocol Fibroblasts were put in RNAlater, Qiagen miRNEasy Mini Kit was used to extract total RNA.
Label Cy3
Label protocol Illumina TotalPrep RNA Amplification Kit was used
 
Hybridization protocol Performed according to Illumina protocol
Scan protocol Illumina Beadstation 500GX according to Illumina protocol
Description Chip sample 4613963056_D
Data processing Cubic spline normalization, subtracted background, False Discovery Rate.
Illumina Beadstudio 3.3 was used with gene expression module 3.4.0
 
Submission date May 05, 2010
Last update date Nov 01, 2011
Contact name Urban Hellman
E-mail(s) urban.hellman@medbio.umu.se
Phone +46907851621
Organization name Umea University
Department Public health and clinical medicine
Street address Nus 6M
City Umea
ZIP/Postal code 90185
Country Sweden
 
Platform ID GPL6885
Series (1)
GSE21676 Cultured fibroblasts treated with media from cardiomyocytes.

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_2896528 285.787 43 14.16533 0
ILMN_2721178 72.10528 39 6.695765 0
ILMN_3033922 1148.971 40 44.81539 0
ILMN_3092673 4367.173 49 125.5274 0
ILMN_2816356 11.09411 28 4.318121 0.09022556
ILMN_2808939 1184.496 36 46.72696 0
ILMN_2634564 375.0959 32 24.81612 0
ILMN_2737647 -2.329347 35 3.079653 0.6015037
ILMN_2734484 156.1697 49 11.70395 0
ILMN_2952292 37.81572 46 4.534319 0.002506266
ILMN_2699078 -1.80119 41 3.1502 0.5651629
ILMN_1213681 205.7716 60 10.23514 0
ILMN_2735413 23.84686 39 6.269957 0.00877193
ILMN_2735415 38.99747 33 5.830343 0.002506266
ILMN_2891688 300.6516 42 19.08129 0
ILMN_2637698 746.2238 36 31.15798 0
ILMN_2674228 468.8221 49 22.64236 0
ILMN_2601546 8.564053 46 3.589054 0.1378446
ILMN_1230831 -0.4827366 31 4.860878 0.4912281
ILMN_2848071 15.3132 36 3.694466 0.04260652

Total number of rows: 25697

Table truncated, full table size 1043 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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