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Sample GSM540785 Query DataSets for GSM540785
Status Public on Nov 01, 2011
Title Fibroblasts NIH-3T3_ultracentrifuged media from cardiomyocytes_rep1
Sample type RNA
 
Source name Fibroblasts_ultracentrifuged media from cardiomyocytes
Organism Mus musculus
Characteristics cell type: fibroblasts
cell line: NIH-3T3
Growth protocol Cultured cardiomyocytes and fibroblasts in claycomb medium in 37 degrees, 5% CO2
Extracted molecule total RNA
Extraction protocol Fibroblasts were put in RNAlater, Qiagen miRNEasy Mini Kit was used to extract total RNA.
Label Cy3
Label protocol Illumina TotalPrep RNA Amplification Kit was used
 
Hybridization protocol Performed according to Illumina protocol
Scan protocol Illumina Beadstation 500GX according to Illumina protocol
Description Chip sample 4936640003_D
Data processing Cubic spline normalization, subtracted background, False Discovery Rate.
Illumina Beadstudio 3.3 was used with gene expression module 3.4.0
 
Submission date May 05, 2010
Last update date Nov 01, 2011
Contact name Urban Hellman
E-mail(s) urban.hellman@medbio.umu.se
Phone +46907851621
Organization name Umea University
Department Public health and clinical medicine
Street address Nus 6M
City Umea
ZIP/Postal code 90185
Country Sweden
 
Platform ID GPL6885
Series (1)
GSE21676 Cultured fibroblasts treated with media from cardiomyocytes.

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_2896528 273.0076 45 9.562275 0
ILMN_2721178 44.55497 51 5.43802 0
ILMN_3033922 1310.635 38 43.4142 0
ILMN_3092673 4477.166 44 145.7634 0
ILMN_2816356 0.09567492 45 4.815933 0.4335839
ILMN_2808939 1635.218 38 56.70705 0
ILMN_2634564 417.02 37 27.65829 0
ILMN_2737647 -2.475905 51 3.803992 0.5814536
ILMN_2734484 118.6635 38 10.87404 0
ILMN_2952292 65.77554 58 5.691936 0
ILMN_2699078 0.5635601 45 4.013283 0.4210526
ILMN_1213681 118.3723 46 7.486202 0
ILMN_2735413 9.898473 43 6.683171 0.1265664
ILMN_2735415 19.03728 31 7.62228 0.03634085
ILMN_2891688 393.3528 36 21.97277 0
ILMN_2637698 866.4733 44 38.88881 0
ILMN_2674228 288.401 43 15.48169 0
ILMN_2601546 16.73832 47 5.743246 0.05263158
ILMN_1230831 -11.41373 35 3.407126 0.9661654
ILMN_2848071 -1.674613 28 3.467893 0.5438597

Total number of rows: 25697

Table truncated, full table size 1041 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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