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Sample GSM540786 Query DataSets for GSM540786
Status Public on Nov 01, 2011
Title Fibroblasts NIH-3T3_ultracentrifuged media from cardiomyocytes_rep2
Sample type RNA
 
Source name Fibroblasts_ultracentrifuged media from cardiomyocytes
Organism Mus musculus
Characteristics cell type: fibroblasts
cell line: NIH-3T3
Growth protocol Cultured cardiomyocytes and fibroblasts in claycomb medium in 37 degrees, 5% CO2
Extracted molecule total RNA
Extraction protocol Fibroblasts were put in RNAlater, Qiagen miRNEasy Mini Kit was used to extract total RNA.
Label Cy3
Label protocol Illumina TotalPrep RNA Amplification Kit was used
 
Hybridization protocol Performed according to Illumina protocol
Scan protocol Illumina Beadstation 500GX according to Illumina protocol
Description Chip sample 4613963056_E
Data processing Cubic spline normalization, subtracted background, False Discovery Rate.
Illumina Beadstudio 3.3 was used with gene expression module 3.4.0
 
Submission date May 05, 2010
Last update date Nov 01, 2011
Contact name Urban Hellman
E-mail(s) urban.hellman@medbio.umu.se
Phone +46907851621
Organization name Umea University
Department Public health and clinical medicine
Street address Nus 6M
City Umea
ZIP/Postal code 90185
Country Sweden
 
Platform ID GPL6885
Series (1)
GSE21676 Cultured fibroblasts treated with media from cardiomyocytes.

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_2896528 232.4861 47 10.87452 0
ILMN_2721178 47.07627 41 6.957343 0
ILMN_3033922 1528.762 49 56.76626 0
ILMN_3092673 5631.874 38 168.4066 0
ILMN_2816356 11.5036 42 3.346242 0.06641604
ILMN_2808939 1533.984 30 47.20753 0
ILMN_2634564 423.502 31 29.78182 0
ILMN_2737647 0.5146908 24 3.54066 0.4298246
ILMN_2734484 139.6012 39 14.56758 0
ILMN_2952292 76.59065 61 5.97056 0
ILMN_2699078 1.398945 50 2.597351 0.3909774
ILMN_1213681 130.4111 54 7.746557 0
ILMN_2735413 25.61797 26 6.054552 0.007518797
ILMN_2735415 33.74356 37 7.129313 0.002506266
ILMN_2891688 310.4913 48 17.20177 0
ILMN_2637698 862.6714 31 42.09285 0
ILMN_2674228 323.5853 47 16.23816 0
ILMN_2601546 8.100565 39 3.815947 0.131579
ILMN_1230831 -4.11065 37 3.455909 0.6754386
ILMN_2848071 2.255098 27 4.157015 0.3471178

Total number of rows: 25697

Table truncated, full table size 1039 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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