|
| Status |
Public on Nov 01, 2011 |
| Title |
Fibroblasts NIH-3T3_microvesicles_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Fibroblasts_microvesicles
|
| Organism |
Mus musculus |
| Characteristics |
cell type: fibroblasts
cell line: NIH-3T3
|
| Growth protocol |
Cultured cardiomyocytes and fibroblasts in claycomb medium in 37 degrees, 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Fibroblasts were put in RNAlater, Qiagen miRNEasy Mini Kit was used to extract total RNA.
|
| Label |
Cy3
|
| Label protocol |
Illumina TotalPrep RNA Amplification Kit was used
|
| |
|
| Hybridization protocol |
Performed according to Illumina protocol
|
| Scan protocol |
Illumina Beadstation 500GX according to Illumina protocol
|
| Description |
Chip sample 4613963056_H
|
| Data processing |
Cubic spline normalization, subtracted background, False Discovery Rate.
Illumina Beadstudio 3.3 was used with gene expression module 3.4.0
|
| |
|
| Submission date |
May 05, 2010 |
| Last update date |
Nov 01, 2011 |
| Contact name |
Urban Hellman |
| E-mail(s) |
urban.hellman@medbio.umu.se
|
| Phone |
+46907851621
|
| Organization name |
Umea University
|
| Department |
Public health and clinical medicine
|
| Street address |
Nus 6M
|
| City |
Umea |
| ZIP/Postal code |
90185 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE21677 |
Cultured fibroblasts treated with microvesicles from cardiomyocytes |
|
