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Sample GSM541178 Query DataSets for GSM541178
Status Public on May 16, 2011
Title Female_Head_4
Sample type RNA
 
Source name Anopheles female head
Organism Anopheles gambiae
Characteristics stage: adult
tissue: head
gender: female
Treatment protocol Mosquitoes were anaesthetised briefly by chilling on ice, then immediately dissected.
Growth protocol 5 day old male and female mosquitoes. Samples for males and females where blooded after the bloodmeal over at three time points over 72hrs (24hrs, 48hr, 72hrs)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol reagent (Invitrogen)
Label biotin
Label protocol Biotinylated cRNA was prepared according to the standard two-cycle Affymetrix protocol from 50ng total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
 
Hybridization protocol Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45C on GeneChip Plasmodium/Anopheles Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip scanner 3000 7G.
Description Anopheles female head
Data processing The data were analyzed with [R] Bioconductor using the GC-RMA normalization and MAS probe detection libraries.
 
Submission date May 05, 2010
Last update date Jan 03, 2012
Contact name Dean Baker
Organization name University of Cambridge
Street address Department of Genetics, Downing Street
City Cambridge
ZIP/Postal code CB2 3EH
Country United Kingdom
 
Platform ID GPL1321
Series (1)
GSE21689 MozAtlas: A comprehensive gene expression atlas of sex- and tissue-specificity in the malaria vector, Anopheles gambiae.

Data table header descriptions
ID_REF
VALUE Normalized GC-RMA Signal Intensity (linear space)
ABS_CALL MAS 5.0 Detection
DETECTION P-VALUE MAS 5.0 Detection p-values

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1116_at 5.265472531 A 0.781016771
1978_at 5.265472531 A 0.988552642
200000_s_at 5.265472531 A 0.570568468
200001_at 5.265472531 A 0.996177074
200002_at 5.265472531 A 0.46457634
200003_s_at 5.266009651 A 0.570568468
200004_at 5.93620214 M 0.045580371
200005_at 5.273111182 A 0.638946097
200006_at 5.265472531 A 0.605162372
200007_at 5.265472531 A 0.856998271
200008_s_at 5.265472531 A 0.671678993
200009_at 6.438535992 A 0.703144167
200010_at 5.308088084 A 0.856998271
200011_s_at 5.265472531 A 0.296855833
200012_x_at 5.272089631 A 0.835967604
200013_at 5.265472531 A 0.979570145
200014_s_at 5.448959864 A 0.605162372
200015_s_at 5.265472531 A 0.954419629
200016_x_at 5.320504382 A 0.638946097
200017_at 8.790324882 A 0.123873163

Total number of rows: 22769

Table truncated, full table size 1002 Kbytes.




Supplementary file Size Download File type/resource
GSM541178_Fhead_53.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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