NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM541195 Query DataSets for GSM541195
Status Public on May 16, 2011
Title Male_Midgut_1
Sample type RNA
 
Source name Anopheles male midgut
Organism Anopheles gambiae
Characteristics stage: adult
tissue: midgut
gender: male
Treatment protocol Mosquitoes were anaesthetised briefly by chilling on ice, then immediately dissected.
Growth protocol 5 day old male and female mosquitoes. Samples for males and females where blooded after the bloodmeal over at three time points over 72hrs (24hrs, 48hr, 72hrs)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol reagent (Invitrogen)
Label biotin
Label protocol Biotinylated cRNA was prepared according to the standard two-cycle Affymetrix protocol from 50ng total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
 
Hybridization protocol Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45C on GeneChip Plasmodium/Anopheles Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip scanner 3000 7G.
Description Anopheles male midgut
Data processing The data were analyzed with [R] Bioconductor using the GC-RMA normalization and MAS probe detection libraries.
 
Submission date May 05, 2010
Last update date Jan 03, 2012
Contact name Dean Baker
Organization name University of Cambridge
Street address Department of Genetics, Downing Street
City Cambridge
ZIP/Postal code CB2 3EH
Country United Kingdom
 
Platform ID GPL1321
Series (1)
GSE21689 MozAtlas: A comprehensive gene expression atlas of sex- and tissue-specificity in the malaria vector, Anopheles gambiae.

Data table header descriptions
ID_REF
VALUE Normalized GC-RMA Signal Intensity (linear space)
ABS_CALL MAS 5.0 Detection
DETECTION P-VALUE MAS 5.0 Detection p-values

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1116_at 5.275617912 A 0.397993682
1978_at 5.275617912 A 0.795977945
200000_s_at 5.275765446 A 0.638946097
200001_at 5.275617912 A 0.969058187
200002_at 5.600543418 A 0.328321007
200003_s_at 7.547710637 A 0.761546785
200004_at 5.275617912 A 0.788201896
200005_at 5.275617912 A 0.945244531
200006_at 5.275617912 A 0.605162372
200007_at 5.275617912 A 0.986884425
200008_s_at 5.275617912 A 0.394837628
200009_at 5.277626207 A 0.211798104
200010_at 5.572956291 A 0.638946097
200011_s_at 5.275617912 A 0.703144167
200012_x_at 5.705673472 A 0.361053903
200013_at 5.275617912 A 0.53542366
200014_s_at 5.275617912 A 0.835967604
200015_s_at 5.275617912 A 0.761546785
200016_x_at 5.644668175 A 0.328321007
200017_at 5.903737599 A 0.106611564

Total number of rows: 22769

Table truncated, full table size 1002 Kbytes.




Supplementary file Size Download File type/resource
GSM541195_Mmidgut_13.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap