|
| Status |
Public on Nov 01, 2011 |
| Title |
Cardiomyocyte microvesicle_DNA content_only one rep. |
| Sample type |
other |
| |
|
| Source name |
Cardiomyocyte microvesicle_DNA content
|
| Organism |
Mus musculus |
| Characteristics |
cell type: cardiomyocyte
cell line: HL-1
|
| Growth protocol |
Cultured cardiomyocytes in claycomb medium in 37 degrees, 5% CO2
|
| Extracted molecule |
other |
| Extraction protocol |
Cardiomyocytes were put in RNAlater, Qiagen miRNEasy Mini Kit was used to extract total RNA. Sigma GenElute Mammalian Genomic DNA Miniprep Kit were used to extract DNA. Terminal deoxynucleotidyl transferase were used to add a poly_T tail to DNA from microvesicles to construct cDNA, possible to label as cRNA and use on the chip to identify part of the sequence of the original DNA.
|
| Label |
Cy3
|
| Label protocol |
Illumina TotalPrep RNA Amplification Kit was used. Terminal deoxynucleotidyl transferase were used to add a poly-T tail to DNA from microvesicles to construct cDNA, possible to label as cRNA and bind to chip.
|
| |
|
| Hybridization protocol |
Performed according to Illumina protocol
|
| Scan protocol |
Illumina Beadstation 500GX according to Illumina protocol
|
| Description |
DNA->cRNA
Chip sample 4936640002_A
|
| Data processing |
Gene expression analysis (not differential) Cubic spline normalization, subtracted background, False Discovery Rate.
Illumina Beadstudio 3.3 was used with gene expression module 3.4.0
|
| |
|
| Submission date |
May 06, 2010 |
| Last update date |
Nov 01, 2011 |
| Contact name |
Urban Hellman |
| E-mail(s) |
urban.hellman@medbio.umu.se
|
| Phone |
+46907851621
|
| Organization name |
Umea University
|
| Department |
Public health and clinical medicine
|
| Street address |
Nus 6M
|
| City |
Umea |
| ZIP/Postal code |
90185 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE21707 |
Identification of cardiomyocyte-derived microvesicles content. |
|
