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Sample GSM542344 Query DataSets for GSM542344
Status Public on May 13, 2010
Title Control_WT_mice_WAT
Sample type RNA
 
Source name C57BL/6 mice, white adipose tissue
Organism Mus musculus
Characteristics genetic background: C57BL/6
gender: female
age: 30-week-old
tissue: perigonadal white adipose tissue
genotype: wild type
Extracted molecule total RNA
Extraction protocol Extraction of total RNA was performed by using Ambion Ribopure Kit (Cat.# AM1924), according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
 
Hybridization protocol Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description Gene expression data from perigonadal white adipose tissue in HFD-fed wildtype C57BL/6 mice.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date May 08, 2010
Last update date May 12, 2010
Contact name Hideaki Miyoshi
URL http://www.med.hokudai.ac.jp/~med-2w/
Organization name Hokkaido University Graduate School of Medicine
Department Internal Medicine II
Street address Kita-15, Nishi-7, Kita-ku
City Sapporo
State/province Hokkaido
ZIP/Postal code 060-8638
Country Japan
 
Platform ID GPL1261
Series (1)
GSE21754 Expression data from white adipose tissue of Perilipin A transgenic mice

Data table header descriptions
ID_REF
VALUE MAS 5.0 signal

Data table
ID_REF VALUE
AFFX-BioB-5_at 683.4
AFFX-BioB-M_at 1032.3
AFFX-BioB-3_at 586.9
AFFX-BioC-5_at 1739.6
AFFX-BioC-3_at 2349.1
AFFX-BioDn-5_at 4112.5
AFFX-BioDn-3_at 5593.3
AFFX-CreX-5_at 20448.7
AFFX-CreX-3_at 22900.1
AFFX-DapX-5_at 1845.8
AFFX-DapX-M_at 3980.7
AFFX-DapX-3_at 4767.5
AFFX-LysX-5_at 344.2
AFFX-LysX-M_at 363.8
AFFX-LysX-3_at 871.1
AFFX-PheX-5_at 436.6
AFFX-PheX-M_at 446.8
AFFX-PheX-3_at 549.1
AFFX-ThrX-5_at 409.7
AFFX-ThrX-M_at 618.7

Total number of rows: 45101

Table truncated, full table size 737 Kbytes.




Supplementary file Size Download File type/resource
GSM542344.CEL.gz 6.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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