|
| Status |
Public on May 13, 2010 |
| Title |
PeriA_Tg_mice_WAT |
| Sample type |
RNA |
| |
|
| Source name |
PeriA transgenic mice, white adipose tissue
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: C57BL/6
gender: female
age: 30-week-old
tissue: perigonadal white adipose tissue
genotype: PeriA transgeneic
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed by using Ambion Ribopure Kit (Cat.# AM1924), according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from perigonadal white adipose tissue in HFD-fed PeriA transgenic mice. The mice are overexpressed human PeriA using the adipocyte specific aP2 promoter/enhancer (Miyoshi, et al. J Lipid Res 2010).
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
May 08, 2010 |
| Last update date |
May 12, 2010 |
| Contact name |
Hideaki Miyoshi |
| URL |
http://www.med.hokudai.ac.jp/~med-2w/
|
| Organization name |
Hokkaido University Graduate School of Medicine
|
| Department |
Internal Medicine II
|
| Street address |
Kita-15, Nishi-7, Kita-ku
|
| City |
Sapporo |
| State/province |
Hokkaido |
| ZIP/Postal code |
060-8638 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE21754 |
Expression data from white adipose tissue of Perilipin A transgenic mice |
|
