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Sample GSM542345 Query DataSets for GSM542345
Status Public on May 13, 2010
Title PeriA_Tg_mice_WAT
Sample type RNA
Source name PeriA transgenic mice, white adipose tissue
Organism Mus musculus
Characteristics genetic background: C57BL/6
gender: female
age: 30-week-old
tissue: perigonadal white adipose tissue
genotype: PeriA transgeneic
Extracted molecule total RNA
Extraction protocol Extraction of total RNA was performed by using Ambion Ribopure Kit (Cat.# AM1924), according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
Hybridization protocol Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description Gene expression data from perigonadal white adipose tissue in HFD-fed PeriA transgenic mice. The mice are overexpressed human PeriA using the adipocyte specific aP2 promoter/enhancer (Miyoshi, et al. J Lipid Res 2010).
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Submission date May 08, 2010
Last update date May 12, 2010
Contact name Hideaki Miyoshi
Organization name Hokkaido University Graduate School of Medicine
Department Internal Medicine II
Street address Kita-15, Nishi-7, Kita-ku
City Sapporo
State/province Hokkaido
ZIP/Postal code 060-8638
Country Japan
Platform ID GPL1261
Series (1)
GSE21754 Expression data from white adipose tissue of Perilipin A transgenic mice

Data table header descriptions
VALUE MAS 5.0 signal

Data table
AFFX-BioB-5_at 626.5
AFFX-BioB-M_at 1042.3
AFFX-BioB-3_at 596.7
AFFX-BioC-5_at 1746.4
AFFX-BioC-3_at 2301.6
AFFX-BioDn-5_at 4076.2
AFFX-BioDn-3_at 5571.3
AFFX-CreX-5_at 20936.3
AFFX-CreX-3_at 23433.3
AFFX-DapX-5_at 1205.9
AFFX-DapX-M_at 3518.8
AFFX-DapX-3_at 4401.3
AFFX-LysX-5_at 249
AFFX-LysX-M_at 388.2
AFFX-LysX-3_at 986.8
AFFX-PheX-5_at 370.4
AFFX-PheX-M_at 458.2
AFFX-PheX-3_at 543.7
AFFX-ThrX-5_at 258.9
AFFX-ThrX-M_at 458.5

Total number of rows: 45101

Table truncated, full table size 736 Kbytes.

Supplementary file Size Download File type/resource
GSM542345.CEL.gz 6.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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