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Sample GSM542374 Query DataSets for GSM542374
Status Public on Jul 01, 2010
Title Parental MEF cells
Sample type RNA
 
Source name Parental MEF
Organism Mus musculus
Characteristics cell type: Parental MEFs
agent: untreated
time: control
Treatment protocol 1.5ug/ml of Dox treatment, if applicable.
Growth protocol Culture of MEFs in standard mouse ES media throughout reprogramming. Cells were split every2-3 days based on density and colony size.
Extracted molecule total RNA
Extraction protocol QIAGEN RNAeasy kit
Label biotin
Label protocol cDNA synthesis was done using Affymetrix WT Kit and in vitro transcription was done using Affymetrix WT IVT Kit
 
Hybridization protocol Samples were hybridized and washed using FS450_0001 protocol
Scan protocol Affymetrix Gene ChIP Scanner 3000
Description Parental MEF cells
MoEx1_020309L_AN08_1 Primary mef.13Dataset_ALL_.rma-gene-core-Signal
Data processing Data was processed using Affymetrix Expression Console at Gene Level of the exon Core probes using RMA normalization.
Gene-level analysis:
probe group file = MoEx-1_0-st-v1.r2.pgf
meta-probeset file = MoEx-1_0-st-v1.r2.dt1.mm9.core.mps
 
Submission date May 10, 2010
Last update date May 10, 2010
Contact name Payman Samavarchi-Tehrani
E-mail(s) payman@lunenfeld.ca
Phone 416-586-4800
Organization name Samuel Lunenfeld Research Institute
Street address 600 University Ave
City Toronto
State/province Ontario
ZIP/Postal code M5G1X5
Country Canada
 
Platform ID GPL6096
Series (1)
GSE21757 Expression data throughout reprogramming of MEF to iPS using a Dox-inducible promoter

Data table header descriptions
ID_REF
VALUE RMA normalized gene level expression values from Expression Console

Data table
ID_REF VALUE
6747308 9.665384
6747309 11.85696
6747314 10.06246
6747326 3.197937
6747343 9.124737
6747354 3.826308
6747364 10.38006
6747471 7.251878
6747472 6.832008
6747478 3.899577
6747497 9.090683
6747504 3.962171
6747515 8.498681
6747577 4.75176
6747641 11.72204
6747696 3.056076
6747786 8.345963
6747805 10.86029
6747837 8.570149
6747839 8.349103

Total number of rows: 23332

Table truncated, full table size 384 Kbytes.




Supplementary file Size Download File type/resource
GSM542374.CEL.gz 22.2 Mb (ftp)(http) CEL
GSM542374.CHP.gz 174.3 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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