|
| Status |
Public on Jul 01, 2010 |
| Title |
1B-Secondary - day5w, day5w/o |
| Sample type |
RNA |
| |
|
| Source name |
1B-secondary cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: 1B-secondary MEFs
agent: Dox (5days), followed by no Dox (5days)
time: day 10
|
| Treatment protocol |
1.5ug/ml of Dox treatment, if applicable.
|
| Growth protocol |
Culture of MEFs in standard mouse ES media throughout reprogramming. Cells were split every2-3 days based on density and colony size.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN RNAeasy kit
|
| Label |
biotin
|
| Label protocol |
cDNA synthesis was done using Affymetrix WT Kit and in vitro transcription was done using Affymetrix WT IVT Kit
|
| |
|
| Hybridization protocol |
Samples were hybridized and washed using FS450_0001 protocol
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000
|
| Description |
1B-secondary - 5days of Dox followed by 5days without Dox
MoEx1_022609H_AN3_+DOX-DOX.13Dataset_ALL_.rma-gene-core-Signal
|
| Data processing |
Data was processed using Affymetrix Expression Console at Gene Level of the exon Core probes using RMA normalization.
Gene-level analysis:
probe group file = MoEx-1_0-st-v1.r2.pgf
meta-probeset file = MoEx-1_0-st-v1.r2.dt1.mm9.core.mps
|
| |
|
| Submission date |
May 10, 2010 |
| Last update date |
May 10, 2010 |
| Contact name |
Payman Samavarchi-Tehrani |
| E-mail(s) |
payman@lunenfeld.ca
|
| Phone |
416-586-4800
|
| Organization name |
Samuel Lunenfeld Research Institute
|
| Street address |
600 University Ave
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G1X5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE21757 |
Expression data throughout reprogramming of MEF to iPS using a Dox-inducible promoter |
|
