|
| Status |
Public on Nov 10, 2010 |
| Title |
2hr_LPS_control_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
LPS-induced macrophages
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 6 weeks old
sex: female
tissue: bone marrow
cell type: macrophages
treatment group: 2hr_LPS_control
|
| Treatment protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| Growth protocol |
Standard culture techniques
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit - followed manufacturer's protocol. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
Data analyses were completed using the R/Bioconductor BioC 2.5 suite of tool. Briefly, raw expression data was quality assessed, and subjected to background adjustment and quantile normalisation using BeadArray. Log2 scaled data was used for linear modelling, differential expression profiling and relevant graphical representations, using the LIMMA library.
|
| |
|
| Submission date |
May 10, 2010 |
| Last update date |
Nov 10, 2010 |
| Contact name |
Paul A Wilson |
| E-mail(s) |
paul.a.wilson@gsk.com
|
| Organization name |
GSK
|
| Department |
Target Sciences
|
| Lab |
Computational Biology
|
| Street address |
GlaxoSmithKline Medicine Research Centre
|
| City |
Stevenage |
| State/province |
Hertfordshire |
| ZIP/Postal code |
SG1 2NY |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE21764 |
To determine the global gene expression response in murine LPS-induced macrophages treated with a bromodomain inhibitor. |
|
