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Sample GSM543027 Query DataSets for GSM543027
Status Public on May 17, 2010
Title MeDIP-Seq analysis of peripheral cd8 naive from TC003 (HS1774)
Sample type SRA
 
Source name HS1774-1
Organism Homo sapiens
Characteristics submitted sample id: JOC41-DNA
donor_id: TC003
Sex: female
body site: Blood
histological type: CD8 naive cell
is tumor: No
biomaterial_type: primary cell
cell_type: CD8 naive cell
Extracted molecule genomic DNA
Extraction protocol library construction protocol: Refer to document 'MeDIP-seq library construction protocol v2' from the Costello Lab at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
 
Library strategy MeDIP-Seq
Library source genomic
Library selection 5-methylcytidine antibody
Instrument model Illumina Genome Analyzer IIx
 
Description design description: MeDIP-Seq analysis of peripheral cd8 naive from TC003 (HS1774) using Illumina Genome Analyzer IIx
library name: HS1774
EXPERIMENT_TYPE: DNA Methylation
EXTRACTION_PROTOCOL: Refer to document 'MeDIP-seq library construction protocol v2' by Costello Lab at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Diagenode Bioruptor
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 25 min
MeDIP_PROTOCOL: Refer to document 'MeDIP-seq library construction protocol v2' by Costello Lab at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
MeDIP_PROTOCOL_DNA_AMOUNT: 2 ug
MeDIP_PROTOCOL_BEAD_TYPE: Protein A/G Beads
MeDIP_PROTOCOL_BEAD_AMOUNT: 100 ul
MeDIP_PROTOCOL_ANTIBODY_AMOUNT: N/A ug
MeDIP_ANTIBODY: Methylcytidine
MeDIP_ANTIBODY_PROVIDER: Eurogentec
MeDIP_ANTIBODY_CATALOG: BI-MECY-0100
MeDIP_ANTIBODY_LOT: 80604
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************
Data processing **********************************************************************

ANALYSIS FILE NAME: GSM543027_UCSF-UBC.CD8-Naive-Primary-Cells.MeDIP-Seq.wig
ANALYSIS ALIAS: UCSF-UBC.CD8-Naive-Primary-Cells.MeDIP-Seq
ANALYSIS TITLE: Raw Signal Density Graphs of CD8 Naive Primary Cells MeDIP-Seq Data
ANALYSIS DESCRIPTION: Illumina MeDIP-Seq read mappings for DNA methylation assessment in CD8 Naive Primary Cells were processed into density graphs of raw signal representing the aligned read density.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.2827
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: MeDIP-Seq
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 0bp
GENOMIC_WINDOW: 20bp
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None

**********************************************************************

ANALYSIS FILE NAME: GSM543027_UCSF-UBC.CD8_Naive_Primary_Cells.MeDIP-Seq.TC003.bed
ANALYSIS ALIAS: HS1774-1_612TYAAXX-HS1774-1_42TCVAAXX.hg19.level.1
ANALYSIS TITLE: Mapping of CD8 Naive Primary Cells MeDIP-Seq Data
ANALYSIS DESCRIPTION: Illumina reads produced by MeDIP-Seq on CD8 Naive Primary Cells, Donor TC003 were mapped to the human genome using Pash.
ANALYSIS TYPE: REFERENCE_ALIGNMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.4244
DATA_ANALYSIS_LEVEL: 1
EXPERIMENT_TYPE: MeDIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: Pash
SOFTWARE_VERSION: 3.0
MAXIMUM_ALIGNMENT_LENGTH: Read length
MISMATCHES_ALLOWED: 10% of read length
ALIGNMENTS_ALLOWED: 1
TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration.
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: None
ALIGNMENT_POSTPROCESSING: None
RELEASE_NUMBER: Human Epigenome Atlas 2


QUALITY SCORES:
NUMBER_OF_MEDIP-SEQ_EXPERIMENTS_SCORED: 24
FINDPEAKS_SCORE: 0.3225
FINDPEAKS_PERCENTILE: 54
HOTSPOT_SCORE: 0.3667
HOTSPOT_PERCENTILE: 58
IROC_SCORE: 0.9637
IROC_PERCENTILE: 70
POISSON_SCORE: 0.6804
POISSON_PERCENTILE: 41

**********************************************************************

ANALYSIS FILE NAME: GSM543027_UCSF-UBC.CD8_Naive_Primary_Cells.MeDIP-Seq.TC003.wig
ANALYSIS ALIAS: HS1774-1_612TYAAXX-HS1774-1_42TCVAAXX.hg19.level.2
ANALYSIS TITLE: Raw Signal Density Graphs of CD8 Naive Primary Cells MeDIP-Seq Data
ANALYSIS DESCRIPTION: Illumina MeDIP-Seq read mappings from CD8 Naive Primary Cells, Donor TC003 were processed into density graphs of raw signal representing the aligned read density.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.4220
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: MeDIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 200bp
GENOMIC_WINDOW: 20bp
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None
RELEASE_NUMBER: Human Epigenome Atlas 2
BROWSER_TRACK_NAME: CD8N MeDIP 03
BROWSER_TRACK_DESCRIPTION: UCSF-UBC-UCD CD8 Naive Primary Cells MeDIP-Seq Donor TC003 EA Release 2


QUALITY SCORES:
NUMBER_OF_MEDIP-SEQ_EXPERIMENTS_SCORED: 24
FINDPEAKS_SCORE: 0.3225
FINDPEAKS_PERCENTILE: 54
HOTSPOT_SCORE: 0.3667
HOTSPOT_PERCENTILE: 58
IROC_SCORE: 0.9637
IROC_PERCENTILE: 70
POISSON_SCORE: 0.6804
POISSON_PERCENTILE: 41

**********************************************************************

 
Submission date May 12, 2010
Last update date May 15, 2019
Contact name UCSF-UBC CENTER
Organization name UCSF-UBC
Street address UCSF-UBC
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
 
Platform ID GPL10999
Series (1)
GSE16368 UCSF-UBC Human Reference Epigenome Mapping Project
Relations
Reanalyzed by GSE69894
Named Annotation GSM543027_UCSF-UBC.CD8_Naive_Primary_Cells.MeDIP-Seq.TC003.wig.gz
Named Annotation GSM543027_UCSF-UBC.CD8-Naive-Primary-Cells.MeDIP-Seq.wig.gz
SRA SRX1157909
BioSample SAMN03416871

Supplementary file Size Download File type/resource
GSM543027_UCSF-UBC.CD8-Naive-Primary-Cells.MeDIP-Seq.wig.gz 56.2 Mb (ftp)(http) WIG
GSM543027_UCSF-UBC.CD8_Naive_Primary_Cells.MeDIP-Seq.TC003.bed.gz 694.8 Mb (ftp)(http) BED
GSM543027_UCSF-UBC.CD8_Naive_Primary_Cells.MeDIP-Seq.TC003.wig.gz 56.2 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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