|
| Status |
Public on Jul 09, 2021 |
| Title |
Small_intestine_irradiated_24h_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Small intestine, irradiated, t=24h
|
| Organism |
Mus musculus |
| Characteristics |
condition: Irradiated
time point: 24 hours
tissue type: Small intestinal tissue
|
| Treatment protocol |
Mice were exposed to 12 gy whole-body ionizing gamma-irradiation, in half of the mice the epithelial fraction was isolated from the small intestine with EDTA.
|
| Growth protocol |
Mice were housed in specific pathogen free conditions, fed regular chow.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using TRI-reagent
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Invitrogen Illumina Totalprep RNA amplification kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Jul 08, 2021 |
| Last update date |
Jul 09, 2021 |
| Contact name |
Sander Meisner |
| E-mail(s) |
s.meisner@amsterdamumc.nl
|
| Phone |
0031205668158
|
| Organization name |
AMC
|
| Department |
Tytgat institute
|
| Lab |
DIGEST
|
| Street address |
Meibergdreef 69-71
|
| City |
Amsterdam |
| State/province |
NH |
| ZIP/Postal code |
1105BK |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE179777 |
Small intestinal tissue and epithelial regeneration after 12 Gy whole-body irradiation of C57BL/6 wild type mice |
|
