NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM544032 Query DataSets for GSM544032
Status Public on Oct 06, 2010
Title canine retina, healthy, biol. rep2
Sample type RNA
 
Source name adult canine retina
Organism Canis lupus familiaris
Characteristics breed: Beagle
age: 4 years
tissue: retina
disease state: healthy
Treatment protocol Canine eyes were enucleated and immediately immersed in RNAlater (Ambion). Eye were stored at -80C until retinal isolation and RNA extraction
Extracted molecule total RNA
Extraction protocol Retina was collected and RNA was extracted using Rneasy columns (Qiagen) including on-column DNA digests
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA
 
Hybridization protocol cRNA was hybridized overnight to Affymetrix Canine 2.0 arrays in a Model 640 hybridization oven. Chips were washed using a Model 450 GeneChip fluidics station.
Scan protocol Data were obtained using a Model 3000 GeneArray scanner.
Description n/a
Data processing RMAexpress
 
Submission date May 17, 2010
Last update date Oct 06, 2010
Contact name Markus H Kuehn
Organization name University of Iowa
Department Ophthalmology and Visual Sciences
Street address 375 Newton Road
City Iowa City
State/province IA
ZIP/Postal code 52242
Country USA
 
Platform ID GPL3738
Series (1)
GSE21879 Neuroinflammation in Advanced Canine Glaucoma

Data table header descriptions
ID_REF
VALUE RMA normalized signal

Data table
ID_REF VALUE
AFFX-BioB-3_at 244.57233
AFFX-BioB-5_at 290.44098
AFFX-BioB-M_at 358.96005
AFFX-BioC-3_at 735.5815
AFFX-BioC-5_at 615.3602
AFFX-BioDn-3_at 3190.341
AFFX-BioDn-5_at 1399.0682
AFFX-Cf_Actin_3_at 5524.968
AFFX-Cf_Actin_5_s_at 1382.1343
AFFX-Cf_Actin_M_at 974.93164
AFFX-Cf_AdrenRecpt_3_at 137.28056
AFFX-Cf_AdrenRecpt_5_at 61.56235
AFFX-Cf_AdrenRecpt_M_at 59.33345
AFFX-Cf_G-6-PDH_3_at 16.94958
AFFX-Cf_G-6-PDH_5_at 42.371483
AFFX-Cf_G-6-PDH_M_at 27.4591
AFFX-Cf_Gapdh_3_at 16050.814
AFFX-Cf_Gapdh_5_at 11595.213
AFFX-Cf_Gapdh_M_at 12934.319
AFFX-Cf_P450-2E1_3_s_at 18.578417

Total number of rows: 43035

Table truncated, full table size 1262 Kbytes.




Supplementary file Size Download File type/resource
GSM544032_Cont2_cf_ret.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap