|
| Status |
Public on Oct 06, 2010 |
| Title |
canine retina, healthy, biol. rep3 |
| Sample type |
RNA |
| |
|
| Source name |
adult canine retina
|
| Organism |
Canis lupus familiaris |
| Characteristics |
breed: Beagle
age: 4 years
tissue: retina
disease state: healthy
|
| Treatment protocol |
Canine eyes were enucleated and immediately immersed in RNAlater (Ambion). Eye were stored at -80C until retinal isolation and RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Retina was collected and RNA was extracted using Rneasy columns (Qiagen) including on-column DNA digests
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA
|
| |
|
| Hybridization protocol |
cRNA was hybridized overnight to Affymetrix Canine 2.0 arrays in a Model 640 hybridization oven. Chips were washed using a Model 450 GeneChip fluidics station.
|
| Scan protocol |
Data were obtained using a Model 3000 GeneArray scanner.
|
| Description |
n/a
|
| Data processing |
RMAexpress
|
| |
|
| Submission date |
May 17, 2010 |
| Last update date |
Oct 06, 2010 |
| Contact name |
Markus H Kuehn |
| Organization name |
University of Iowa
|
| Department |
Ophthalmology and Visual Sciences
|
| Street address |
375 Newton Road
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL3738 |
| Series (1) |
| GSE21879 |
Neuroinflammation in Advanced Canine Glaucoma |
|
