|
Status |
Public on Jan 11, 2022 |
Title |
M-CSF donor 3 |
Sample type |
RNA |
|
|
Source name |
blood
|
Organism |
Homo sapiens |
Characteristics |
stimulation: LPS-stimulated
|
Treatment protocol |
On day 7, cells were activated by 10 ng/ml lipopolysaccharide (LPS).
|
Growth protocol |
Human monocytes were isolated from the blood using Milteny negative separation kit. Monocytes were differentiated into macrophages and dendritic cells for 7 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using Trizol and further precipitated using ammonium acetate.
|
Label |
biotin
|
Label protocol |
Standard Illumina protocol
|
|
|
Hybridization protocol |
Standard Illumina protocol (Illumina Hybridiation Oven Model 5521)
|
Scan protocol |
Illumina iScan scanner
|
Description |
M-CSF donor 3
|
Data processing |
The data were background corrected and quantile normalized using the neqc function from the limma R package (v3.46.0).
|
|
|
Submission date |
Jul 14, 2021 |
Last update date |
Jan 11, 2022 |
Contact name |
Manuela Rossol |
E-mail(s) |
manuela.rossol@medizin.uni-leipzig.de
|
Organization name |
University of Leipzig
|
Lab |
Experimental Rheumatology
|
Street address |
Liebigstr. 19
|
City |
Leipzig |
ZIP/Postal code |
04103 |
Country |
Germany |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE180111 |
Danger signal initiates differentiation of monocytes into osteopontin‑producing macrophages II |
|