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Sample GSM5453380 Query DataSets for GSM5453380
Status Public on Jan 11, 2022
Title M-CSF donor 3
Sample type RNA
 
Source name blood
Organism Homo sapiens
Characteristics stimulation: LPS-stimulated
Treatment protocol On day 7, cells were activated by 10 ng/ml lipopolysaccharide (LPS).
Growth protocol Human monocytes were isolated from the blood using Milteny negative separation kit. Monocytes were differentiated into macrophages and dendritic cells for 7 days.
Extracted molecule total RNA
Extraction protocol RNA was isolated using Trizol and further precipitated using ammonium acetate.
Label biotin
Label protocol Standard Illumina protocol
 
Hybridization protocol Standard Illumina protocol (Illumina Hybridiation Oven Model 5521)
Scan protocol Illumina iScan scanner
Description M-CSF
donor 3
Data processing The data were background corrected and quantile normalized using the neqc function from the limma R package (v3.46.0).
 
Submission date Jul 14, 2021
Last update date Jan 11, 2022
Contact name Manuela Rossol
E-mail(s) manuela.rossol@medizin.uni-leipzig.de
Organization name University of Leipzig
Lab Experimental Rheumatology
Street address Liebigstr. 19
City Leipzig
ZIP/Postal code 04103
Country Germany
 
Platform ID GPL10558
Series (1)
GSE180111 Danger signal initiates differentiation of monocytes into osteopontin‑producing macrophages II

Data table header descriptions
ID_REF
VALUE quantile

Data table
ID_REF VALUE
ILMN_1343291 7.67918867869544
ILMN_1343295 9.08964137996026
ILMN_1651228 13.0622028797509
ILMN_1651229 7.199701596756
ILMN_1651254 8.83783731252966
ILMN_1651259 4.7885475817644
ILMN_1651262 4.10105762853906
ILMN_1651278 4.10884271092393
ILMN_1651282 4.1140934648871
ILMN_1651315 9.96627389313104
ILMN_1651336 4.09963056545522
ILMN_1651341 4.11010546294956
ILMN_1651343 4.32125478690816
ILMN_1651346 6.47737618667824
ILMN_1651347 8.29814957129091
ILMN_1651354 8.83601938153831
ILMN_1651369 4.12764050077076
ILMN_1651378 8.6179627937959
ILMN_1651385 4.11825270571074
ILMN_1651403 4.11053094970631

Total number of rows: 15526

Table truncated, full table size 453 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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