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Sample GSM545785 Query DataSets for GSM545785
Status Public on Jun 13, 2010
Title 18-32 nt total small RNAs (Mov10l-/-)
Sample type SRA
 
Source name Testes
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: Mov10l-/- mutant
age: 10 dpp
antibody: Total small RNAs
Treatment protocol Testes were extracted from euthanized animals of indicated ages
Growth protocol Mice were maintained and genotyped as per standard procedures
Extracted molecule total RNA
Extraction protocol Mov10L1 was immunoprecipitated from mouse testes extracts, small RNAs recovered by phenol-choloroform extraction, 5'-end labelled with gamma-ATP and gel extracted.
Total RNA was separated by 15% Urea-PAGE and RNAs in the range of 18-32 nt were gel extracted. RNA libraries were prepared with Digital Gene Expression for Small RNA kit from Illumina.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection size fractionation
Instrument model Illumina Genome Analyzer
 
Description Mov10l-associated small RNAs and total 18-32 nt small RNAs extracted after PAGE separation.
This sample represents total small RNAs
Data processing In-house programs developed by Alexander Stark and Ravi Sachidanandam. Barcoded dataset (5' GUUCAGAGUUCUACAGUCCGACGAUCCGAC; barcode CGAC is at 3' end) was first sorted into respective libraries and then mapped to the genome (July 2007, mm9 assembly).
 
Submission date May 20, 2010
Last update date May 15, 2019
Contact name Ramesh Pillai
E-mail(s) ramesh.pillai@unige.ch
Organization name University of Geneva
Department Department of Molecular Biology
Street address 30, Quai Ernest-Ansermet
City Gneveva
ZIP/Postal code CH-1211
Country Switzerland
 
Platform ID GPL9185
Series (1)
GSE21763 Mouse MOV10L1 associates with Piwi proteins and is an essential component of the piRNA pathway
Relations
SRA SRX021366
BioSample SAMN00014098

Data table header descriptions
SEQUENCE
COUNT readcount

Data table
SEQUENCE COUNT
AAAAAAAAGGCAAACTTAACCGACACG 1
AAAAAAAATTGGAACGATACAGAGAAG 1
AAAAAAAGCCGGACGTGGTGGCGCAC 2
AAAAAAAGCTGGGCAGTGGTGGCACA 1
AAAAAAATTGGAACGATACAGAGAAGA 1
AAAAAAATTGTCCAGGACTCTCTGCTTGT 1
AAAAAACATTAGACTGTG 2
AAAAAACCAGTCGGGTCCTTGACCAAG 1
AAAAAAGAGGAAAGAAGGAAAAA 1
AAAAAAGCCGGGCGTGGTGGCGCA 1
AAAAAAGGAAAGGCGGG 1
AAAAAAGGAGGGGCTGGAGAGATGGC 1
AAAAAATACACTGAACAGA 1
AAAAAATTGGAACGATACAGAGAAG 1
AAAAACACATTCGTTGGAA 1
AAAAACAGAGACAGTATACTCACATACAT 1
AAAAACAGCTGCAGTGTAC 1
AAAAACAGTTCTCTGCTTGT 1
AAAAACATGAACTCCTGAG 1
AAAAACTCCGGACTGGCGCAGGAGG 1

Total number of rows: 37487

Table truncated, full table size 934 Kbytes.




Supplementary data files not provided
SRA Run SelectorHelp
Processed data included within Sample table
Raw data are available in SRA

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