|
| Status |
Public on Jun 25, 2010 |
| Title |
Ago2ko_ES_rep7 |
| Sample type |
SRA |
| |
|
| Source name |
embryonic stem cells
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Ago2 -/-
cell type: embryonic stem cells
|
| Treatment protocol |
100nM 4-OH-tamoxifen for 4-9 days for the Drosha experiment
|
| Growth protocol |
Standard tissue culture practice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Global 5`-phosphate-dependent RACE in WT, Ago2-KO and Drosha-excised mouse ES cells and human 293S cells
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
5`-phosphorylated poly(A) RNA
|
| Data processing |
n/a
|
| |
|
| Submission date |
May 24, 2010 |
| Last update date |
Mar 04, 2023 |
| Contact name |
Fedor Karginov |
| E-mail(s) |
karginov@ucr.edu
|
| Organization name |
UC Riverside
|
| Department |
MCSB
|
| Street address |
900 University Ave
|
| City |
Riverside |
| State/province |
California |
| ZIP/Postal code |
92521 |
| Country |
USA |
| |
|
| Platform ID |
GPL9250 |
| Series (1) |
| GSE21975 |
Diverse endonucleolytic cleavage sites in the mammalian transcriptome depend upon microRNAs, Drosha, and additional nucleases |
|
| Relations |
| SRA |
SRX021458 |
| BioSample |
SAMN00014158 |
