|
| Status |
Public on May 01, 2019 |
| Title |
wild-type mice treated with AICAR_A |
| Sample type |
RNA |
| |
|
| Source name |
liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB
genotype/variation: wild-type
agent: AICAR
age: 10 wks
gender: male
tissue: liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
WT AICAR-A
Biological replicate 1
|
| Data processing |
The data were normalized using cubic spline in Illumina GenomeStudio and also using the VAMPIRE Bayesian model with a 2-sided Bonferroni-corrected threshold of 0.05.
|
| |
|
| Submission date |
May 25, 2010 |
| Last update date |
May 01, 2019 |
| Contact name |
Ruth T Yu |
| E-mail(s) |
yu@salk.edu
|
| Phone |
858-453-4100
|
| Organization name |
Salk Institute
|
| Street address |
10010 N. Torrey Pines Rd.
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE21984 |
The LKB1/AMPK pathway is a central mediator of the hepatic xenobiotic response |
|
