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Sample GSM546685 Query DataSets for GSM546685
Status Public on May 01, 2019
Title LKB liver-specific knockout mice treated with AICAR_B
Sample type RNA
 
Source name liver
Organism Mus musculus
Characteristics strain: FVB
genotype/variation: LKB1 lox/lox
agent: AICAR
age: 10 wks
gender: male
tissue: liver
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description LKBko AICAR-B
Biological replicate 2
Data processing The data were normalized using cubic spline in Illumina GenomeStudio and also using the VAMPIRE Bayesian model with a 2-sided Bonferroni-corrected threshold of 0.05.
 
Submission date May 25, 2010
Last update date May 01, 2019
Contact name Ruth T Yu
E-mail(s) yu@salk.edu
Phone 858-453-4100
Organization name Salk Institute
Street address 10010 N. Torrey Pines Rd.
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platform ID GPL6885
Series (1)
GSE21984 The LKB1/AMPK pathway is a central mediator of the hepatic xenobiotic response

Data table header descriptions
ID_REF
VALUE quantile-normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1212607 157.0636 0.3809524
ILMN_1212612 212.959 0.01002506
ILMN_1212619 136.0597 0.9749373
ILMN_1212628 149.2 0.6629072
ILMN_1212632 284.4148 0
ILMN_1212636 3807.738 0
ILMN_1212637 3619.698 0
ILMN_1212645 8885.281 0
ILMN_1212648 512.1177 0
ILMN_1212653 200.9731 0.02255639
ILMN_1212672 770.2491 0
ILMN_1212682 150.137 0.6090226
ILMN_1212683 157.3022 0.3746867
ILMN_1212685 141.8719 0.8972431
ILMN_1212692 199.307 0.02631579
ILMN_1212693 227.9978 0.005012531
ILMN_1212695 161.6338 0.2919799
ILMN_1212698 138.6002 0.9486216
ILMN_1212702 10100.45 0
ILMN_1212703 444.357 0

Total number of rows: 25697

Table truncated, full table size 723 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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