|
| Status |
Public on Apr 05, 2003 |
| Title |
Spleen vs CD27 Treated B cell 30min (set2) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Spleen was labeled with Cyanine-3 (green).
|
| Organism |
Mus musculus |
| Extracted molecule |
total RNA |
| |
|
| Channel 2 |
| Source name |
CD27 Treated B cells were labeled with Cyanine-5 (red)
|
| Organism |
Mus musculus |
| Extracted molecule |
total RNA |
| |
|
| |
| Description |
total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CD27 for 30min
|
| |
|
| Submission date |
Apr 04, 2003 |
| Last update date |
May 27, 2005 |
| Contact name |
Sangdun Choi |
| E-mail(s) |
schoi@its.caltech.edu
|
| Phone |
626-395-8732
|
| URL |
http://www.signaling-gateway.org
|
| Organization name |
California Institute of Technology
|
| Department |
Biology
|
| Lab |
Alliance for Cellular Signaling Molecular Biology Laboratory
|
| Street address |
1200 E. California Blvd
|
| City |
Pasadena |
| State/province |
CA |
| ZIP/Postal code |
91125 |
| Country |
USA |
| |
|
| Platform ID |
GPL260 |
| Series (2) |
| GSE375 |
Spleen vs CD27 Treated B cell |
| GSE2050 |
Major Patterns of B Cell Gene Expression Changes in Response to Short-Term Stimulation with 33 Single Ligands |
|
| Data table header descriptions |
| ID_REF |
Feature number which correpond to the order of genes on the array |
| RATIO |
Raw Log10 Ratio |
| G_PROCESSED |
Green Processed signal, after dye normalization and background subtraction, used for computation of log ratio |
| R_PROCESSED |
Red Processed signal, after dye normalization and background subtraction, used for computation of log ratio |
| G_MEAN |
Green Channel Mean Intensity |
| R_MEAN |
Red Channel Mean Intensity |
| G_MEDIAN |
Green Channel Median Intensity |
| R_MEDIAN |
Red Channel Median Intensity |
| G_SD |
Green Channel Standard Deviation |
| R_SD |
Red Channel Standard Deviation |
| G_BG_MEAN |
Green Channel Mean Background Level |
| R_BG_MEAN |
Red Channel Mean Background Level |
| G_BG_MEDIAN |
Green Channel Median Background Level |
| R_BG_MEDIAN |
Red Channel Median Background Level |
| G_BG_SD |
Green Channel Background Standard Deviation |
| R_BG_SD |
Red Channel Background Standard Deviation |
| VALUE |
Processed Log2 Ratio after removing saturated spots, spot below 2.6XSD of background, and nonuniform spots. |
