NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM5497 Query DataSets for GSM5497
Status Public on Apr 05, 2003
Title Spleen vs CD27 Treated B cell 120min (set1)
Sample type RNA
 
Channel 1
Source name Spleen was labeled with Cyanine-3 (green).
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name CD27 Treated B cells were labeled with Cyanine-5 (red)
Organism Mus musculus
Extracted molecule total RNA
 
 
Description total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with CD27 for 120min
 
Submission date Apr 04, 2003
Last update date May 27, 2005
Contact name Sangdun Choi
E-mail(s) schoi@its.caltech.edu
Phone 626-395-8732
URL http://www.signaling-gateway.org
Organization name California Institute of Technology
Department Biology
Lab Alliance for Cellular Signaling Molecular Biology Laboratory
Street address 1200 E. California Blvd
City Pasadena
State/province CA
ZIP/Postal code 91125
Country USA
 
Platform ID GPL260
Series (2)
GSE375 Spleen vs CD27 Treated B cell
GSE2050 Major Patterns of B Cell Gene Expression Changes in Response to Short-Term Stimulation with 33 Single Ligands

Data table header descriptions
ID_REF Feature number which correpond to the order of genes on the array
RATIO Raw Log10 Ratio
G_PROCESSED Green Processed signal, after dye normalization and background subtraction, used for computation of log ratio
R_PROCESSED Red Processed signal, after dye normalization and background subtraction, used for computation of log ratio
G_MEAN Green Channel Mean Intensity
R_MEAN Red Channel Mean Intensity
G_MEDIAN Green Channel Median Intensity
R_MEDIAN Red Channel Median Intensity
G_SD Green Channel Standard Deviation
R_SD Red Channel Standard Deviation
G_BG_MEAN Green Channel Mean Background Level
R_BG_MEAN Red Channel Mean Background Level
G_BG_MEDIAN Green Channel Median Background Level
R_BG_MEDIAN Red Channel Median Background Level
G_BG_SD Green Channel Background Standard Deviation
R_BG_SD Red Channel Background Standard Deviation
VALUE Processed Log2 Ratio after removing saturated spots, spot below 2.6XSD of background, and nonuniform spots.

Data table
ID_REF RATIO G_PROCESSED R_PROCESSED G_MEAN R_MEAN G_MEDIAN R_MEDIAN G_SD R_SD G_BG_MEAN R_BG_MEAN G_BG_MEDIAN R_BG_MEDIAN G_BG_SD R_BG_SD VALUE
1 -0.779433 58759 9764.31 65517 15046.4 65517 13875 0.69282 3615.93 826.124 915.609 828 920 29.4735 37.092 0
2 -0.0746532 147.126 123.89 941.56 1057.08 933 1038 53.5024 74.3028 813.384 906.679 813 906 24.948 36.2352 0
3 0.135204 30.6692 41.8703 820.88 940.88 814 942 36.4992 37.6157 814.922 910.647 813 912 21.1485 41.0217 0
4 -0.298417 41.3433 20.7964 825.458 891.708 827 902.5 23.335 48.5964 810.897 902.935 811 900 23.9206 37.9413 0
5 0 29.4703 23.4943 807.913 892.043 811 897 18.7978 38.4276 812.096 903.86 812 902 24.8479 44.5501 0
6 -0.0329256 450.815 417.901 1290.11 1457.25 1302 1466.5 117.325 148.957 812.669 897.841 811 896 23.0292 38.3995 0
7 0.111325 28.0878 36.2946 810.667 922.708 809 917.5 18.4053 46.362 809.268 901.366 806 902 24.913 40.7658 0
8 -0.165883 44.0506 30.0655 819.593 902.074 816 904 31.2315 47.6896 801.519 892.875 801 895.5 20.9254 39.6919 0
9 0.180189 6232.26 9437.02 7978.77 13935 7981.5 13962.5 418.799 829.372 812.831 896.694 812 894.5 24.7211 40.4242 0.598574901
10 0.612583 1013.62 4153.91 1946.2 6649.32 1904 5713 366.866 2657.01 805.656 901.3 803 898 23.8547 42.303 0
11 0 28.7897 26.4548 814.737 890 813 888 10.6815 32.1057 811.47 885.841 809 886 25.2428 47.481 0
12 0 29.56 26.8773 801.923 893.231 799.5 892 20.3763 32.1102 799.915 888.216 800 890 25.8729 43.6078 0
13 0.0798332 26.174 31.4559 796.08 919.72 793 922 23.0361 46.9552 800.203 905.941 798 904 22.7508 40.3183 0
14 0.227401 1076.58 1817.39 2009.46 3437.36 2069 3487 188.742 427.601 811.361 916.899 811 917.5 22.444 39.8678 0.755409771
15 0.0857433 2920.71 3558.21 4158.43 5784.86 2497 3605 3090.9 4453.86 800.184 913.006 801 909.5 22.4127 32.8962 0
16 0.334085 1436.82 3100.9 2425.62 5187.27 2106.5 4669 834.146 1984.71 798.497 911.02 798 910 21.4541 33.0443 0
17 -0.416601 822.684 315.234 1687.91 1302.13 1598 1289 360.576 129.976 793.071 893.814 789.5 895 24.0178 37.2607 -1.383918566
18 0.0662623 540.656 629.772 1384.12 1735.27 1388.5 1733.5 70.7227 98.9135 801.792 878.058 796.5 881 29.2129 42.834 0.220118596
19 0.404189 4694.53 11906.4 6200.88 17334 6329.5 17978.5 625.027 2148.66 806.157 879.203 805 877 22.9069 39.1032 1.342686795
20 -0.0848158 5892.96 4847.5 7605.17 7549.13 7521.5 7460.5 865.892 868.523 809.098 894.327 811 887 21.1948 41.1545 -0.281751989

Total number of rows: 16273

Table truncated, full table size 1979 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap