|
| Status |
Public on Feb 08, 2011 |
| Title |
H3K4me1.rep2.s_2 |
| Sample type |
SRA |
| |
|
| Source name |
H3K4me1.rep2.s_2
|
| Organism |
Mus musculus |
| Characteristics |
developmental stage: P5
tissue type: 4 P5 cerebella (pooled)
genotype: Atoh1FLAG/FLAG
sequencing protocol: single-end, 37-mer sequencing
chip antibody: Histone H3 (mono methyl K4)
antibody manufacturer: Abcam
antibody catalog number: AB8895
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was MNase digested and subject to ChIP using the indicated antibody. The recovered fragmented DNA was end polished and ligated with Illumina methylated PE adaptors. Sizes between 200 bp (including the adaptors) were selected and sequenced with the Illumina Genome Analyzer II.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
chromatin IP against Histone H3K4me1
|
| Data processing |
Reads are mapped to genome (mm9) using ELAND with up to 2 mismatches allowed.
|
| |
|
| Submission date |
Jun 03, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Yuanxin Xi |
| E-mail(s) |
xiyuanxin@yahoo.com
|
| Phone |
530-220-2067
|
| Organization name |
The University of Texas MD Anderson Cancer Center
|
| Department |
Bioinformatics and Computational Biology
|
| Street address |
1400 Pressler St
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL9250 |
| Series (1) |
| GSE22111 |
The Atoh1 targetome in murine postnatal cerebellum |
|
| Relations |
| SRA |
SRX022869 |
| BioSample |
SAMN00016817 |
