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Sample GSM550514 Query DataSets for GSM550514
Status Public on Jun 04, 2010
Title Rottweiler_Joey_hemangiosarcoma
Sample type RNA
 
Source name hemangiosarcoma tumor cell line
Organism Canis lupus familiaris
Characteristics breed: Rottweiler
id: Joey
tumor type: hemangiosarcoma
tumor origin: spleen
Growth protocol Cryopreserved cultured cells from the earliest available passages were used or primary tumor cells
Extracted molecule total RNA
Extraction protocol RNA was prepared using the RNEasy Mini Kit and QIAshredder columns (QIAGEN, Valencia, CA)
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Canine 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Gene expression data from dogs with hemangiosarcoma of different breeds
Data processing The data were background corrected with the RMA algorithm using R and the Bioconductor's affy package. All parameters were set at its default settings
 
Submission date Jun 04, 2010
Last update date Jun 04, 2010
Contact name Beth Tamburini
E-mail(s) beth.tamburini@ucdenver.edu
Organization name University of Colorado Denver
Department Immunology
Street address 1400 Jackson Street, K503
City Denver
State/province CO
ZIP/Postal code 80206
Country USA
 
Platform ID GPL3738
Series (2)
GSE22129 Gene Expression Profiling Identifies Inflammation and Angiogenesis as Distinguishing Features of Canine Hemangiosarcoma
GSE23760 Gene Expression Profiling Identifies Inflammation and Angiogenesis as Distinguishing Features of Canine Hemangiosarcoma

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 6.666496941
AFFX-BioB-5_at 6.984107521
AFFX-BioB-M_at 6.973750681
AFFX-BioC-3_at 8.29147014
AFFX-BioC-5_at 8.103603942
AFFX-BioDn-3_at 10.62626005
AFFX-BioDn-5_at 9.244632718
AFFX-Cf_Actin_3_at 13.10247211
AFFX-Cf_Actin_5_s_at 12.92079379
AFFX-Cf_Actin_M_at 12.48841495
AFFX-Cf_AdrenRecpt_3_at 6.108961123
AFFX-Cf_AdrenRecpt_5_at 5.119741476
AFFX-Cf_AdrenRecpt_M_at 4.797898507
AFFX-Cf_G-6-PDH_3_at 3.017781032
AFFX-Cf_G-6-PDH_5_at 4.433617396
AFFX-Cf_G-6-PDH_M_at 3.743319375
AFFX-Cf_Gapdh_3_at 13.54752696
AFFX-Cf_Gapdh_5_at 13.18089242
AFFX-Cf_Gapdh_M_at 13.39839895
AFFX-Cf_P450-2E1_3_s_at 2.750986075

Total number of rows: 43035

Table truncated, full table size 1356 Kbytes.




Supplementary file Size Download File type/resource
GSM550514.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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