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Sample GSM550786 Query DataSets for GSM550786
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 2
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 57
gender: F
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 738.5379
AFFX-BioB-M_at 1098.14307
AFFX-BioB-3_at 1087.28821
AFFX-BioC-5_at 2224.11353
AFFX-BioC-3_at 1606.30432
AFFX-BioDn-5_at 3476.68164
AFFX-BioDn-3_at 5663.04004
AFFX-CreX-5_at 7825.27197
AFFX-CreX-3_at 9079.19434
AFFX-DapX-5_at 31.64687
AFFX-DapX-M_at 5.76264
AFFX-DapX-3_at 17.21958
AFFX-LysX-5_at 13.4247
AFFX-LysX-M_at 11.13102
AFFX-LysX-3_at 12.35343
AFFX-PheX-5_at 19.13715
AFFX-PheX-M_at 14.16419
AFFX-PheX-3_at 28.65631
AFFX-ThrX-5_at 9.84764
AFFX-ThrX-M_at 6.16424

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550786.CEL.gz 5.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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