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Sample GSM550787 Query DataSets for GSM550787
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 1
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 68
gender: F
gold stage: Stage III
group: Severe
plateid: 1
batch: A
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 901.81305
AFFX-BioB-M_at 1329.4718
AFFX-BioB-3_at 1291.99585
AFFX-BioC-5_at 2588.59839
AFFX-BioC-3_at 1910.27161
AFFX-BioDn-5_at 3986.21509
AFFX-BioDn-3_at 6149.91504
AFFX-CreX-5_at 8264.99707
AFFX-CreX-3_at 9243.63574
AFFX-DapX-5_at 49.36295
AFFX-DapX-M_at 8.86157
AFFX-DapX-3_at 33.26276
AFFX-LysX-5_at 8.83714
AFFX-LysX-M_at 20.18791
AFFX-LysX-3_at 14.9172
AFFX-PheX-5_at 17.87011
AFFX-PheX-M_at 7.77811
AFFX-PheX-3_at 22.46893
AFFX-ThrX-5_at 2.50671
AFFX-ThrX-M_at 22.24792

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550787.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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