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Sample GSM550790 Query DataSets for GSM550790
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 5
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 65
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 1166.099
AFFX-BioB-M_at 1732.72974
AFFX-BioB-3_at 1632.78418
AFFX-BioC-5_at 2916.59204
AFFX-BioC-3_at 2284.22388
AFFX-BioDn-5_at 4042.64185
AFFX-BioDn-3_at 6139.0498
AFFX-CreX-5_at 7809.55273
AFFX-CreX-3_at 8739.77051
AFFX-DapX-5_at 33.02277
AFFX-DapX-M_at 5.32067
AFFX-DapX-3_at 21.15561
AFFX-LysX-5_at 14.87064
AFFX-LysX-M_at 1.27018
AFFX-LysX-3_at 9.42677
AFFX-PheX-5_at 9.50688
AFFX-PheX-M_at 19.5383
AFFX-PheX-3_at 34.80839
AFFX-ThrX-5_at 6.06257
AFFX-ThrX-M_at 24.3751

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550790.CEL.gz 4.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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