NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM550791 Query DataSets for GSM550791
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 6
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 60
gender: F
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 1117.78516
AFFX-BioB-M_at 1687.27368
AFFX-BioB-3_at 1600.13684
AFFX-BioC-5_at 2964.80151
AFFX-BioC-3_at 2291.72021
AFFX-BioDn-5_at 4244.23633
AFFX-BioDn-3_at 6415.7666
AFFX-CreX-5_at 8047.42334
AFFX-CreX-3_at 9844.7207
AFFX-DapX-5_at 48.46844
AFFX-DapX-M_at 7.3045
AFFX-DapX-3_at 19.38746
AFFX-LysX-5_at 10.23419
AFFX-LysX-M_at 8.26315
AFFX-LysX-3_at 8.88832
AFFX-PheX-5_at 18.02617
AFFX-PheX-M_at 11.12401
AFFX-PheX-3_at 32.41914
AFFX-ThrX-5_at 14.21773
AFFX-ThrX-M_at 21.72159

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550791.CEL.gz 4.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap