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Sample GSM550792 Query DataSets for GSM550792
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 7
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 62
gender: M
gold stage: Stage II
group: Moderate
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 778.58856
AFFX-BioB-M_at 1194.18518
AFFX-BioB-3_at 1148.54541
AFFX-BioC-5_at 2292.56543
AFFX-BioC-3_at 1677.25562
AFFX-BioDn-5_at 3458.26611
AFFX-BioDn-3_at 5884.52148
AFFX-CreX-5_at 7727.76855
AFFX-CreX-3_at 8851.37207
AFFX-DapX-5_at 32.68335
AFFX-DapX-M_at 5.82603
AFFX-DapX-3_at 17.19922
AFFX-LysX-5_at 11.27895
AFFX-LysX-M_at 8.09833
AFFX-LysX-3_at 11.45227
AFFX-PheX-5_at 18.8313
AFFX-PheX-M_at 15.44811
AFFX-PheX-3_at 25.39979
AFFX-ThrX-5_at 13.30371
AFFX-ThrX-M_at 20.23915

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550792.CEL.gz 5.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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