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Sample GSM550793 Query DataSets for GSM550793
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 2
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 60
gender: M
gold stage: Stage III
group: Severe
plateid: 1
batch: A
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 802.8927
AFFX-BioB-M_at 1207.92456
AFFX-BioB-3_at 1128.15039
AFFX-BioC-5_at 2255.06982
AFFX-BioC-3_at 1672.92004
AFFX-BioDn-5_at 3326.15259
AFFX-BioDn-3_at 5648.53613
AFFX-CreX-5_at 7790.66113
AFFX-CreX-3_at 8575.34277
AFFX-DapX-5_at 30.0851
AFFX-DapX-M_at 14.12663
AFFX-DapX-3_at 18.37873
AFFX-LysX-5_at 15.35572
AFFX-LysX-M_at 9.41742
AFFX-LysX-3_at 6.22816
AFFX-PheX-5_at 8.21483
AFFX-PheX-M_at 10.30309
AFFX-PheX-3_at 22.3375
AFFX-ThrX-5_at 8.26677
AFFX-ThrX-M_at 18.36449

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550793.CEL.gz 5.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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